Ancymidol (Synonyms: EL 531) |
Katalog-Nr.GC48874
One-Click Copy Product Info
|
Ancymidol is a biologically active pyrimidine-class plant growth regulator.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 12771-68-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Ancymidol is a biologically active pyrimidine-class plant growth regulator. Ancymidol can block the abnormal elongation of plant cells by inhibiting the oxidation of gibberellin biosynthetic intermediates, while also suppressing plant growth by interfering with cellulose biosynthesis. Ancymidol can be used in related research in plant pathology and mycology[1-4].
In vitro, maize suspension-cultured cells were treated with Ancymidol (12.5μM–2mM) for 8 days. Ancymidol significantly inhibited cell growth and induced a globular cell shape, while reducing cellulose deposition in the cell wall and promoting xylan synthesis as a compensatory response[5]. Phaseolus vulgaris seedlings and Gibberella fujikuroi cultures were treated with Ancymidol (10–100μg/ml). Ancymidol significantly inhibited the endogenous gibberellin biosynthesis and the gibberellin-like activity in the extracts; Phaseolus vulgaris seedlings cultured in the dark were treated with Ancymidol (10μg/ml) for 0 to 4 days. Ancymidol significantly induced or enhanced the production of compounds that inhibited the leaf sheath elongation of Oryza sativa[6].
References:
[1] Coolbaugh RC, Hirano SS, West CA. Studies on the Specificity and Site of Action of alpha-Cyclopropyl-alpha-[p-methoxyphenyl]-5-pyrimidine Methyl Alcohol (Ancymidol), a Plant Growth Regulator. Plant Physiol. 1978 Oct;62(4):571-6.
[2] Chen J, Ziv M. The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep. 2001 Jan;20(1):22-27.
[3] Feng XR, Wolyn DJ. Development of haploid asparagus embryos from liquid cultures of anther-derived calli is enhanced by ancymidol. Plant Cell Rep. 1993 Mar;12(5):281-5.
[4] Li B, Wolyn DJ. The effects of ancymidol, abscisic acid, uniconazole and paclobutrazol on somatic embryogenesis of asparagus. Plant Cell Rep. 1995 May;14(8):529-33.
[5] Hernández-Altamirano JM, Largo-Gosens A, Martínez-Rubio R, et al. Effect of ancymidol on cell wall metabolism in growing maize cells. Planta. 2018 Apr;247(4):987-999.
[6] Shive JB, Sisler HD. Effects of Ancymidol (a Growth Retardant) and Triarimol (a Fungicide) on the Growth, Sterols, and Gibberellins of Phaseolus vulgaris (L.). Plant Physiol. 1976 Apr;57(4):640-4.
| Cell experiment [1]: | |
Cell lines | Maize (Zea mays L. Black Mexican sweet corn) suspension-cultured cells |
Preparation Method | Maize cells were maintained in Murashige and Skoog (MS) medium supplemented with 10μM 2,4-D and 20g/L sucrose (pH=5.6), at 25°C under photoperiodic conditions (16:8 light/dark cycle; 41μmol/m²/s) with rotary shaking (110rpm), and routinely subcultured fortnightly. Ancymidol (dissolved in ethanol) was applied at varying concentrations immediately after subculturing. In reversal experiments, 500μM Ancymidol-treated cultures were supplemented with 100μM GA₃. |
Reaction Conditions | 12.5μM to 2mM; 8 days |
Applications | Ancymidol concentrations ≤500μM progressively reduced cell growth and induced a globular cell shape without affecting cell viability; the I₅₀ value for the inhibitory effect on fresh weight gain was 658μM, and this growth inhibition was reversed by supplementation with 100μM GA₃. Ancymidol impaired cellulose accumulation in cell walls, as 500μM Ancymidol caused reduction in cellulose content and reduced the incorporation of [¹⁴C]glucose into α-cellulose, which was not reversed by the simultaneous application of GA₃. Ancymidol increased the incorporation of [³H]arabinose into a hemicellulose-enriched (4M KOH) fraction and up-regulated ZmIRX9 and ZmIRX10L gene expression, indicating an enhancement in arabinoxylan biosynthesis as a compensatory response to cellulose reduction. |
References: | |
| Cas No. | 12771-68-5 | SDF | |
| Überlieferungen | EL 531 | ||
| Canonical SMILES | OC(C1=CC=C(C=C1)OC)(C2CC2)C3=CN=CN=C3 | ||
| Formula | C15H16N2O2 | M.Wt | 256.3 |
| Löslichkeit | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:7): 0.12 mg/ml,Ethanol: 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.9017 mL | 19.5084 mL | 39.0168 mL |
| 5 mM | 780.3 μL | 3.9017 mL | 7.8034 mL |
| 10 mM | 390.2 μL | 1.9508 mL | 3.9017 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)