Boc-Lys(Ac)-AMC |
Katalog-Nr.GC11641
One-Click Copy Product Info
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Boc-Lys(Ac)-AMC is a substrate of histone deacetylases (HDACs).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 233691-67-3
Sample solution is provided at 25 µL, 10mM.
Boc-Lys(Ac)-AMC is a substrate of histone deacetylases (HDACs). HDACs catalyze the deacetylation of Boc-Lys(Ac)-AMC and through addition of a trypsin-containing developer, the deacetylated product can convert to a quantifiable fluorophore (Ex/Em=355nm/460nm) [1].
References:
[1] Reddy D S, Wu X, Golub V M, et al. Measuring histone deacetylase inhibition in the brain [J]. Current protocols in pharmacology, 2018, 81(1): e41.
This plan only provides a guide, please modify it to meet your specific needs.
Ⅰ. Tissue culture-based HDAC assays [1]
1. Seed 6×104 cells in 100μl medium in 96-well tissue culture plates and incubate it overnight.
2. Add 25μM HDAC substrate Boc-Lys(Ac)-AMC into the overnight culture and incubate it at 37℃ with 5% CO2 atmosphere for 2~3h.
3. Add HDAC developer solution containing cell lysis buffer and trypsin reagents to terminate the deacetylation reaction.
4. After 15min incubation at room temperature, measure the fluorescent signal of AMC by microplate reader at Ex/Em=355nm/460nm.
References:
[1] Ueki N, Lee S, Sampson N S, et al. Selective cancer targeting with prodrugs activated by histone deacetylases and a tumour-associated protease [J]. Nature Communications, 2013, 4(1): 2735.
| Cas No. | 233691-67-3 | SDF | |
| Chemical Name | (S)-tert-butyl (6-acetamido-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxohexan-2-yl)carbamate | ||
| Canonical SMILES | CC(C)(C)OC(N[C@H](C(NC(C=C1)=CC(O2)=C1C(C)=CC2=O)=O)CCCCNC(C)=O)=O | ||
| Formula | C23H31N3O6 | M.Wt | 445.51 |
| Löslichkeit | ≥ 16.2mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2446 mL | 11.2231 mL | 22.4462 mL |
| 5 mM | 448.9 μL | 2.2446 mL | 4.4892 mL |
| 10 mM | 224.5 μL | 1.1223 mL | 2.2446 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)