Catalpol (Synonyms: Catalpinoside, 7,8-epoxy Aucubin) |
Katalog-Nr.GN10094
One-Click Copy Product Info
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Catalpol is an iridoid glycoside found in Rehmannia glutinosa, which has neuroprotective, anti-diabetic, anti-inflammatory, antioxidant and antiviral effects.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2415-24-9
Sample solution is provided at 25 µL, 10mM.
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Catalpol is an iridoid glycoside found in Rehmannia glutinosa, which has neuroprotective, anti-diabetic, anti-inflammatory, antioxidant and antiviral effects[1, 2]. Catalpol can activate AMP-activated protein kinase to regulate lipid metabolism and reduce hepatic steatosis[3].
In vitro, Catalpol (1, 10, 100μM) treatment of human induced pluripotent stem cells (iPSCs) for 24 h increased cell viability in a dose-dependent manner and reduced intracellular lactate dehydrogenase (LDH) levels and lipid peroxidation levels[4]. Catalpol (10μM) treatment of oligodendrocyte precursor cells (OPCs) promoted OPC proliferation, cell migration and differentiation into mature oligodendrocytes[5].
In vivo, Catalpol (5, 10, 20mg/kg) was intraperitoneally injected into gerbils with cerebral ischemia-reperfusion injury for 3 days, which significantly improved the stroke index, significantly increased brain tissue SOD activity, reduced MDA content, and reduced plasma ET-1 content[6]. Catalpol (25-100mg/kg) was injected intraperitoneally into mice with renal ischemia-reperfusion injury, which reduced the blood urea nitrogen and serum creatinine levels, the expression of PI3K, Akt and eNOS, and inhibited the activity of TNF-α, IL-1β, IL-6 and IL-10[7].
References:
[1] Xia Y, Lu Y W, Hao R J, et al. Catalpol relieved angiotensin II-induced blood–brain barrier destruction via inhibiting the TLR4 pathway in brain endothelial cells[J]. Pharmaceutical Biology, 2022, 60(1): 2210-2218.
[2] Bai Y, Zhu R, Tian Y, et al. Catalpol in diabetes and its complications: a review of pharmacology, pharmacokinetics, and safety[J]. Molecules, 2019, 24(18): 3302.
[3] Tian X, Ru Q, Xiong Q, et al. Catalpol attenuates hepatic steatosis by regulating lipid metabolism via AMP‐activated protein kinase activation[J]. BioMed Research International, 2020, 2020(1): 6708061.
[4] Yang L, Feng X, Li Y, et al. Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells[J]. AMB Express, 2020, 10(1): 56.
[5] Yuan C X, Chu T, Liu L, et al. Catalpol induces oligodendrocyte precursor cell-mediated remyelination in vitro[J]. American Journal of Translational Research, 2015, 7(11): 2474.
[6] Liu Y, Li P, Suo J, et al. Catalpol provides protective effects against cerebral ischaemia/reperfusion injury in gerbils[J]. Journal of Pharmacy and Pharmacology, 2014, 66(9): 1265-1270.
[7] Zhu J, Chen X, Wang H, et al. Catalpol protects mice against renal ischemia/reperfusion injury via suppressing PI3K/Akt-eNOS signaling and inflammation[J]. International Journal of Clinical and Experimental Medicine, 2015, 8(2): 2038.
| Cell experiment [1]: | |
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Cell lines |
Human-induced pluripotent stem cells (iPSCs) |
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Preparation Method |
The study included five groups: Group I, normal control, human iPSCs incubated with dimethyl sulfoxide (DMSO) for 24h; Group II, treatment control, human iPSCs incubated with 8 µM aconitine for 24h; Group III, low-dose treatment, human iPSCs incubated with 8 µM aconitine and 1µM Catalpol for 24h; Group IV, medium-dose treatment, human iPSCs incubated with 8µM aconitine and 10µM Catalpol for 24h; and Group V, high-dose treatment, human iPSCs incubated with 8µM aconitine and 100µM Catalpol for 24h. |
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Reaction Conditions |
1, 10, 100μM; 24h |
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Applications |
Compared with the treatment control group, Catalpol supplementation (1, 10, and 100µM) increased iPSC cell viability by 7.5, 27.3, and 65.8%, respectively; reduced the LDH levels by 10.4, 31.3, and 75.2%, respectively; and reduced the lipid peroxidation levels by 7.7, 33.0, and 62.6%, respectively. |
| Animal experiment [2]: | |
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Animal models |
Mongolian gerbils |
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Preparation Method |
A gerbil model of CI/R was prepared by bilateral common carotid occlusion for 10min followed by 6h reperfusion. Catalpol (5, 10 or 20mg/kg per day) was injected intraperitoneally for 3 days before the carotid occlusion. Stroke index was measured during the reperfusion. The contents of endogenous neuropeptides, endothelin-1 (ET-1) and calcitonin gene-related peptide in plasma were evaluated by radioimmunoassay. Superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue homogenate were also examined. |
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Dosage form |
5, 10, 20mg/kg; 3 days; i.p. |
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Applications |
Catalpol significantly improved the stroke index compared with CI/R control group. Catalpol significantly increased the activity of SOD at the doses of 10 and 20mg/kg, decreased the brain MDA content and the plasma level of ET-1 at the doses of 10 and 20mg/kg. |
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References: |
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| Cas No. | 2415-24-9 | SDF | |
| Überlieferungen | Catalpinoside, 7,8-epoxy Aucubin | ||
| Chemical Name | (2S,3R,4S,5S,6R)-2-[[(1aS,1bS,2S,5aR,6S,6aS)-6-hydroxy-1a-(hydroxymethyl)-2,5a,6,6a-tetrahydro-1bH-oxireno[5,6]cyclopenta[1,3-c]pyran-2-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol | ||
| Canonical SMILES | C1=COC(C2C1C(C3C2(O3)CO)O)OC4C(C(C(C(O4)CO)O)O)O | ||
| Formula | C15H22O10 | M.Wt | 362.33 |
| Löslichkeit | DMF: 30 mg/ml,DMSO: 30 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7599 mL | 13.7996 mL | 27.5991 mL |
| 5 mM | 552 μL | 2.7599 mL | 5.5198 mL |
| 10 mM | 276 μL | 1.38 mL | 2.7599 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)