Difopein |
Katalog-Nr.GC16682
One-Click Copy Product Info
|
14.3.3 protein inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 396834-58-5
Sample solution is provided at 25 µL, 10mM.
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Difopein is an inhibitor of 14-3-3 protein.
14-3-3 protein has been involved in a wide range of biologic processes such as signal transduction, vesicular transport, cell cycle control, DNA replication, DNA repair and apoptosis. 14-3-3 protein has been associated with tumor genesis. 14-3-3 protein correlates with reduced tumor cell apoptosis and abbreviated patient survival. Up-regulation of 14-3-3 protein provides a survival advantage to tumor cells. Inhibition of 14-3-3 protein might trigger apoptosis, inhibit growth of established tumors, suppress de novo tumor formation, and promise a novel therapeutic approach for glioma treatment [1].
In human glioma U251 and U87 cells, difopein resulted in apoptosis characteristics, such as nuclear fragmentation, DNA laddering fragment and appearance of membrane-enclosed apoptotic bodies. Difopein induced apoptosis in a time-dependent manner [1]. Difopein induced glioma cell apoptosis through down-regulating Bcl-2, up-regulating Bax and activating caspase-9 and caspase-3. In vivo, difopein effectively hindered proliferation and triggered apoptosis of tumor cells implanted into nude mice [1].
Reference:
[1] Cao W, Yang X, Zhou J, et al. Targeting 14-3-3 protein, difopein induces apoptosis of human glioma cells and suppresses tumor growth in mice[J]. Apoptosis, 2010, 15(2): 230-241.
| Cas No. | 396834-58-5 | SDF | |
| Formula | C273H424N76O89S6 | M.Wt | 6387.17 |
| Löslichkeit | Soluble to 5 mg/ml in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 156.6 μL | 782.8 μL | 1.5656 mL |
| 5 mM | 31.3 μL | 156.6 μL | 313.1 μL |
| 10 mM | 15.7 μL | 78.3 μL | 156.6 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: ≥90.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)