Bind-Silane |
Katalog-Nr.GF16032
One-Click Copy Product Info
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Bind-Silane is a silane reagent with glass-affinity modification activity.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Bind-Silane is a silane reagent with glass-affinity modification activity. Bind-Silane forms a binding layer on clean long glass plates through silanization, enabling polyacrylamide gels to firmly adhere to glass plates and preventing detachment during gel casting, electrophoresis, staining, and drying. Bind-Silane is used for SDS-PAGE, native PAGE, sequencing polyacrylamide gel preparation, protein or nucleic acid gel glass plate treatment, and laboratory glass surface modification[1-4].
References:[1] Zeng HL, Li H, Wang X, et al. Chiral separation of FITC-labeled amino acids with gel electrochromatography using a polydimethylsiloxane microfluidic device. J Capill Electrophor Microchip Technol. 2007;10(1-2):19-24.
[2] Barberi R, Bonvent JJ, Bartolino R,et al. Probing soft polymeric coatings of a capillary by atomic force microscopy. J Chromatogr B Biomed Appl. 1996 Aug 9;683(1):3-13.
[3] Zeng HL, Li HF, Wang X, et al. Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation. Talanta. 2006 Mar 15;69(1):226-31.
[4] Quandt N, Stindl A, Keller U. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis for M(r) estimations of high-molecular-weight polypeptides. Anal Biochem. 1993 Nov 1;214(2):490-4.
Bind-Silane for polyacrylamide gel adhesion experimental protocol[1]
This experimental protocol is compiled based on the research content for reference only. Please adjust according to actual needs.
(1) Reagent preparation: Prepare Bind-Silane solution. Prepare 1M NaOH solution. Prepare detergent, deionized water, and ethanol.
(2) Glass plate pretreatment: Soak long and short glass plates in 1M NaOH for a period of time. Clean with detergent once. Rinse off detergent with water. Rinse thoroughly with deionized water. Finally, wipe glass plates with ethanol.
(3) Long glass plate treatment: Before each gel casting, dip a lens tissue in approximately 1mL Bind-Silane solution (depending on glass plate size) and apply to the long glass plate. Coat once from top to bottom and once from left to right to cover the entire glass plate evenly. After 5-10min, gently wipe the glass plate with clean lens tissue to remove excess Bind-Silane. Alternatively, wash the long glass plate 3 times with 95% ethanol to remove excess Bind-Silane.
(4) Short glass plate treatment: Before each gel casting, change gloves. Dip a lens tissue in approximately 1.5mL Repel-Silane solution (depending on glass plate size) and apply to the short glass plate. Coat once from top to bottom and once from left to right to cover the entire glass plate evenly. After 5-10min, gently wipe the glass plate with clean lens tissue to remove excess Repel-Silane.
(5) Reuse treatment: If glass plates need to be reused, soak in 1M NaOH overnight and then rinse with plenty of water.
Precautions:
(1) Gloves must be changed before treating the short glass plate to prevent Bind-Silane on gloves from contaminating the short glass plate and causing adhesion.
(2) Bind-Silane is only for treating long glass plates to make gels adhere. Repel-Silane is only for treating short glass plates to facilitate gel separation.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:[1] Quandt N, Stindl A, Keller U. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis for M(r) estimations of high-molecular-weight polypeptides. Anal Biochem. 1993 Nov 1;214(2):490-4.
| Cas No. | SDF | ||
| Formula | M.Wt | ||
| Löslichkeit | Storage | Store at RT | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)