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4-Methylumbelliferyl-β-D-Glucopyranoside (Synonyms: 4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU)

カタログ番号GC11870

4-メチルウンベリフェリル-β-D-グルコピラノシド、β-D-グルコシドは、β-グルコシダーゼの蛍光基質であり、β-グルコシダーゼ活性のアッセイに利用されます。

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4-Methylumbelliferyl-β-D-Glucopyranoside 化学構造

Cas No.: 18997-57-4

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10mM (in 1mL DMSO)
$142.00
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1g
$127.00
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5g
$430.00
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Sample solution is provided at 25 µL, 10mM.

Description of 4-Methylumbelliferyl-β-D-Glucopyranoside

4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease.1,2,3 Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4

References:

1. Hernández-Guzmán, A., Flores-Martínez, A., Ponce-Noyola, P., et al. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii FEBS Open Bio. 6(11), 1067-1077 (2016).
2. Kwapiszewski, R., Skolimowski, M., Ziółkowska, K., et al. A microfluidic device with fluorimetric detection for intracellular components analysis Biomed Microdevices 13(3), 431-440 (2011).
3. Shanmuganathan, M.K., and Britz-McKibbin, P. Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis Anal. Bioanal. Chem. 399(8), 2843-2853 (2011).
4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule J. Spectrosc. 1(1), 147128 (2013).

Chemical Properties of 4-Methylumbelliferyl-β-D-Glucopyranoside

Cas No. 18997-57-4 SDF
同義語 4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU
Chemical Name 4-methyl-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2H-chromen-2-one
Canonical SMILES OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O1
Formula C16H18O8 M.Wt 338.3
溶解度 ≥ 84.5mg/mL in DMSO with gentle warming Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of 4-Methylumbelliferyl-β-D-Glucopyranoside

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.956 mL 14.7798 mL 29.5596 mL
5 mM 0.5912 mL 2.956 mL 5.9119 mL
10 mM 0.2956 mL 1.478 mL 2.956 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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