Phleomycin (Synonyms: Zeocin) |
カタログ番号GC14081
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Phleomycinは糖ペプチドの抗生物質とDNA鎖切断(DNA-SB)の誘導剤であり、遺伝子組換え細胞の選択に使われることが可能である。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11006-33-0
Sample solution is provided at 25 µL, 10mM.
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Phleomycinは糖ペプチドの抗生物質とDNA鎖切断(DNA-SB)の誘導剤であり、遺伝子組換え細胞の選択に使われることが可能である[1]。Phleomycinはストレプトマイセス属に発見された分子であり、ブレオマイシンに似ていて、DNAに結合、挿入でき、二重螺旋構造の完全性を阻害する[2]。Phleomycinは耐性連鎖球菌株に対し、変異原性作用を持つ[3]。
試験管内、Phleomycin (100-400μg/mL)でHeLa細胞を0-24時間処理した結果、時間と用量依存的に細胞の増殖を阻害し、細胞毒性を誘発し、サイトクロムcの最初の放出、Bcl-XL、ENDOG、DAXXとMDM2の下方制御、そしてCASPとBIDの上方制御を誘発した[4]。
Phleomycin (0.2μg/mL)でナンノクロロプシス・オーセアニカ細胞を48時間処理した結果、藻類細胞のDNAでランダムな二本鎖切断を起こし、活性ハイグロマイシンB耐性遺伝子（aph7）の変換効率を増やした[5]。様々な変異体からのマウス胎児線維芽細胞（MEF）をPhleomycin (0-200ng/mL)で48時間処理した結果、MEFを欠けるXRCC4、DNA-Ligase IVまたはXlfの生存率を著しく減らしたが、PAXXからのMEFと野生型のMEFに対してはこれと言った効果はなかった[6]。
References:
[1] Ma X, Pan K, Zhang L, et al. Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker[J]. Journal of Ocean University of China, 2016, 15: 351-356.
[2] Stokowa-Sołtys K, Dzyhovskyi V, Wieczorek R, et al. Phleomycin complex–Coordination mode and in vitro cleavage of DNA[J]. Journal of inorganic biochemistry, 2019, 195: 71-82.
[3] Van Peer A F, De Bekker C, Vinck A, et al. Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune[J]. Applied and Environmental Microbiology, 2009, 75(5): 1243-1247.
[4] Hwang J, Kim Y Y, Huh S, et al. The Time‐Dependent Serial Gene Response to Zeocin Treatment Involves Caspase‐Dependent Apoptosis in HeLa Cells[J]. Microbiology and immunology, 2005, 49(4): 331-342.
[5] Zhang Z, Guo L, Liu H, et al. Zeocin treatment significantly elevated transformation efficiency of Nannochloropsis oceanica[J]. Journal of Applied Phycology, 2022, 34(3): 1587-1594.
[6] Abramowski V, Etienne O, Elsaid R, et al. PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice[J]. Cell Death & Differentiation, 2018, 25(2): 444-452.
| 細胞実験[1]: | |
細胞株 | HeLa細胞 |
準備方法 | 細胞（1ウェルあたり6×104）を24ウェルプレートに1日間付着させ、Phleomycin を添加し、0, 6 , 12, 18, 24時間で、100, 200, 300, 400μg/mLのPhleomycinで血球計数器で測定した。HeLa細胞に対するPhleomycinの細胞毒性作用を、生細胞の数とMTTアッセイで測定した。 |
反応条件 | 100, 200, 300, 400μg/mL；0-24時間 |
アプリケーション | Phleomycinは用量と時間依存的にHeLa細胞の増殖を阻害し、細胞毒性を誘発する。 |
References: | |
| Cas No. | 11006-33-0 | SDF | |
| 同義語 | Zeocin | ||
| Chemical Name | (2R,3S,4S,5R,6R)-2-(((2R,3S,4S,5S,6S)-2-(2-(6-amino-2-(3-amino-1-((2,3-diamino-3-oxopropyl)amino)-3-oxopropyl)-5-methylpyrimidine-4-carboxamido)-3-((5-((1-((2-(4-((4-guanidinobutyl)carbamoyl)-4',5'-dihydro-[2,4'-bithiazol]-2'-yl)ethyl)amino)-3-hydroxy-1-o | ||
| Canonical SMILES | NC(CNC(CC(N)=O)C1=NC(N)=C(C(C(NC(C(NC(C(C(C)C(NC(C(NCCC2=NC(C3=NC(C(NCCCCNC(N)=N)=O)=CS3)CS2)=O)C(O)C)=O)O)C)=O)C(O[C@H]4[C@]([H])([C@H]([C@@H]([C@@H](O4)CO)O)O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@@H]5O)OC(N)=O)O)CO)C6=CNC=N6)=O)=N1)C)C(N)=O.[Cu+2].Cl | ||
| Formula | C55H85N20O21S2Cu • HCl | M.Wt | 1526.5 |
| 溶解度 | ≤10mg/ml in PBS, pH 7.2 | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 655.1 μL | 3.2755 mL | 6.5509 mL |
| 5 mM | 131 μL | 655.1 μL | 1.3102 mL |
| 10 mM | 65.5 μL | 327.5 μL | 655.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)