Phleomycin (Synonyms: Zeocin) |
Catalog No.GC14081
One-Click Copy Product Info
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Phleomycin은 사카로펩타이드 항생물질이고 DNA 사슬단열 (DNA-SB) 유도체로 사용되어 유전 공학 세포를 선택하는 데 사용할 수 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11006-33-0
Sample solution is provided at 25 µL, 10mM.
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Phleomycin은 사카로펩타이드 항생물질이고 DNA 사슬단열 (DNA-SB) 유도체로 사용되어 유전 공학 세포를 선택하는 데 사용할 수 있다. Phleomycin은 스트렙토마이세스에서 발견된 분자이고 블레오마이신과 유사하게 DNA에 결합하여 삽입하여 이중 나선 구조의 완전성을 방해한다[2]. Phleomycin은 내성 연쇄상구균 균주에 돌연변이 효과가 있다[3].
체외 실험에서 HeLa 세포를 Phleomycin (100-400μg/mL)으로 0-24시간 처리하면 세포 증식을 시간 및 농도 의존적으로 억제하고 세포 독성을 유도하고 세포색소 c의 초기 방출을 유도하고 Bcl-XL, ENDOG, DAXX 및 MDM2의 인하과 CASP 및 BID의 인상을 일으킨다[4]. Nannochloropsis oceanica 세포를 Phleomycin (0.2μg/mL)으로 48시간 처리하면 해조류 세포의 DNA에 랜덤한 이중 사슬 단열을 일으키고 활성 하이그로마이신 B 저항성 유전자 (aph7)의 변환 효율을 증가시킨다[5]. Phleomycin (0-200ng/mL)으로 48시간 처리한 다른 돌연변이체에서 유래한 쥐 배아 섬유아세포 (MEFs)의 생존률은 XRCC4, DNA-Ligase IV 또는 Xlf가 결핍된 MEFs에서 유의하게 감소되었지만 PAXX 돌연변이체 및 야생형 MEFs에는 유의한 영향을 미치지 않았다[6].
References:
[1] Ma X, Pan K, Zhang L, et al. Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker[J]. Journal of Ocean University of China, 2016, 15: 351-356.
[2] Stokowa-Sołtys K, Dzyhovskyi V, Wieczorek R, et al. Phleomycin complex–Coordination mode and in vitro cleavage of DNA[J]. Journal of inorganic biochemistry, 2019, 195: 71-82.
[3] Van Peer A F, De Bekker C, Vinck A, et al. Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune[J]. Applied and Environmental Microbiology, 2009, 75(5): 1243-1247.
[4] Hwang J, Kim Y Y, Huh S, et al. The Time‐Dependent Serial Gene Response to Zeocin Treatment Involves Caspase‐Dependent Apoptosis in HeLa Cells[J]. Microbiology and immunology, 2005, 49(4): 331-342.
[5] Zhang Z, Guo L, Liu H, et al. Zeocin treatment significantly elevated transformation efficiency of Nannochloropsis oceanica[J]. Journal of Applied Phycology, 2022, 34(3): 1587-1594.
[6] Abramowski V, Etienne O, Elsaid R, et al. PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice[J]. Cell Death & Differentiation, 2018, 25(2): 444-452.
| 세포 실험 [1]: | |
세포 라인 | HeLa 세포 |
제조 방법 | 세포 (웰당 6×104)는 24웰 판에서 1일 동안 부착을 허용하고 Phleomycin을 추가한 후 0, 6, 12, 18, 24시간 및 100, 200, 300, 400μg/mL의 Phleomycin에서 혈구계를 사용하여 문화를 측정합니다. Phleomycin의 HeLa 세포에 대한 세포 독성 효과는 생존 세포 수와 MTT 시험으로 결정됩니다. |
반응 조건 | 100, 200, 300, 400μg/mL; 0-24시간 동안 |
응용 분야 | Phleomycin은 농도 및 시간 의존적으로 HeLa 세포의 증식을 억제하고 세포 독성을 유도합니다. |
References: | |
| Cas No. | 11006-33-0 | SDF | |
| Synonyms | Zeocin | ||
| Chemical Name | (2R,3S,4S,5R,6R)-2-(((2R,3S,4S,5S,6S)-2-(2-(6-amino-2-(3-amino-1-((2,3-diamino-3-oxopropyl)amino)-3-oxopropyl)-5-methylpyrimidine-4-carboxamido)-3-((5-((1-((2-(4-((4-guanidinobutyl)carbamoyl)-4',5'-dihydro-[2,4'-bithiazol]-2'-yl)ethyl)amino)-3-hydroxy-1-o | ||
| Canonical SMILES | NC(CNC(CC(N)=O)C1=NC(N)=C(C(C(NC(C(NC(C(C(C)C(NC(C(NCCC2=NC(C3=NC(C(NCCCCNC(N)=N)=O)=CS3)CS2)=O)C(O)C)=O)O)C)=O)C(O[C@H]4[C@]([H])([C@H]([C@@H]([C@@H](O4)CO)O)O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@@H]5O)OC(N)=O)O)CO)C6=CNC=N6)=O)=N1)C)C(N)=O.[Cu+2].Cl | ||
| Formula | C55H85N20O21S2Cu • HCl | M.Wt | 1526.5 |
| Solubility | ≤10mg/ml in PBS, pH 7.2 | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 655.1 μL | 3.2755 mL | 6.5509 mL |
| 5 mM | 131 μL | 655.1 μL | 1.3102 mL |
| 10 mM | 65.5 μL | 327.5 μL | 655.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)