Phalloidin (Synonyms: NSC 523214) |
| Catalog No.GC15692 |
Phalloidin은 형광색소(Λex=495 nm, Λem=520 nm)로 세포골격의 F-액틴을 표지하는 데 사용할 수 있는 버섯 유래 독소입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17466-45-4
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm).
Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].
References:
[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *