12-o-tetradecanoylphorbol-13-acetate (TPA, PMA)
Within the field of normal disquisition, certain mixes play a critical portion in unleashing the bigwig data of cellular forms and understanding complex common fabrics. Chemical mixes have continuously charmed experimenters with their one of a kind packages and empirical operations.
Cycloheximide
Produced by the bacterium Streptomyces griseus, Cycloheximide is a glutarimide antibiotic, occupying a unique space in the scientific tool box due to its antifungal properties and potential role in the inhibition of protein synthesis.
Phos-tag biotin BTL-104
A monobiotinylated Phos-tag derivative called BTL-104 is used to identify phosphopeptides and phosphoproteins. When the ligand molecule and biotin combine to form a functional conjugate (Phos-tag Biotin BTL-104), phosphorylated analytes on a PVDF membrane can be seen, making Phos-tagTM a potent tool.
Phos-tag Acrylamide
A useful chemical called Phos-tagTM is capable of capturing phosphorylated His/Asp/Lys and Ser/Thr/Tyr. It is a synthetic chemical molecule that was created using the catalytic domain of alkaline phosphatase as a model. Phos-tagged proteins can be separated, detected, analyzed using mass spectrometry, and purified using a range of procedures that use Phos-tagTM. An innovative and useful method for the enrichment and analysis of phosphorylated proteins is offered by phospho-tag acrylamide,
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