D-Allose |
Katalog-Nr.GC62921
One-Click Copy Product Info
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D-Allose ist ein kÖrpereigener Metabolit.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2595-97-3
Sample solution is provided at 25 µL, 10mM.
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D-Allose is an ultra-low-calorie aldohexose that exhibits cryoprotective function on cell survival during freezing [1]. D-Allose protects mouse hippocampal HT-22 cells from oxygen-glucose deprivation and reperfusion (OGD/R) -induced injury by inhibiting the Gal-3/TLR4 signalling pathway [2]. D-Allose has been widely used to inhibit the proliferation of a variety of cancer cells and prevent cancer cells from taking up glucose[3].
In vitro, D-Allose treatment for 48 hours significantly inhibited the viability of MIA PaCa-2 cells with an IC50 value of 53.25mM[4]. Treatment of cells with 40mM D-Allose for 48 hours inhibited the growth of PC-3 cells, induced apoptosis, and caused cell cycle arrest[5]. Treatment of RT112 cells with 50mM D-Allose for 24h inhibited cell viability, induced intracellular reactive oxygen species (ROS) production, and stimulated TXNIP expression[6].
In vivo, D-Allose treatment via intraperitoneal injection at a dose of 100mg/kg/day for 28 days significantly reduced tumor volume in a subcutaneous U87MG cell xenograft mouse model [7]. Intravenous administration of 0.4mg/g D-Allose 1 hour before ischemia significantly inhibited apoptosis, reduced blood brain barrier (BBB) permeability, and decreased inflammatory cytokine levels in the mice model of middle cerebral artery occlusion and reperfusion (MCAO/Rep) [8].
References:
[1] Chen Z, Chen J, Zhang W, et al. Recent research on the physiological functions, applications, and biotechnological production of D-allose[J]. Applied microbiology and biotechnology, 2018, 102(10): 4269-4278.
[2] Huang Y, Thonusin C, Tokuda M, et al. The beneficial effects of D-allose and D-allulose on the brain under ischemic stroke and obese-insulin resistant conditions: evidence from in vitro to clinical studies[J]. Metabolic Brain Disease, 2025, 40(4): 1-15.
[3] Noguchi C, Kamitori K, Hossain A, et al. D-allose inhibits cancer cell growth by reducing GLUT1 expression[J]. The Tohoku journal of experimental medicine, 2016, 238(2): 131-141.
[4] Malm S W, Hanke N T, Gill A, et al. The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines[J]. Journal of Experimental & Clinical Cancer Research, 2015, 34(1): 31.
[5] Naha N, Lee H Y, Jo M J, et al. Rare sugar D-allose induces programmed cell death in hormone refractory prostate cancer cells[J]. Apoptosis, 2008, 13(9): 1121-1134.
[6] Tohi Y, Taoka R, Zhang X, et al. Antitumor effects of orally administered rare sugar D-allose in bladder cancer[J]. International Journal of Molecular Sciences, 2022, 23(12): 6771.
[7] Suzuki K, Ogawa D, Kanda T, et al. Antiproliferative effects of D-allose associated with reduced cell division frequency in glioblastoma[J]. Scientific Reports, 2023, 13(1): 19515.
[8] Huang T, Gao D, Hei Y, et al. D-allose protects the blood brain barrier through PPARγ-mediated anti-inflammatory pathway in the mice model of ischemia reperfusion injury[J]. Brain research, 2016, 1642: 478-486.
| Cell experiment [1]: | |
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Cell lines |
MIA PaCa-2 cells |
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Preparation Method |
MIA PaCa-2 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum and 4.5g/l glucose. Cells were seeded in 96-well plates at a density of 3000 cells/well, with the lateral wells left empty for water addition. Cells were treated with different concentrations of D-Allose (0.01, 0.1, 1,10, and 100mM) for 48 hours, and cell proliferation was analyzed. |
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Reaction Conditions |
0.01, 0.1, 1,10, and 100mM; 48h |
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Applications |
D-Allose treatment decreased the cell viability of MIA PaCa-2 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Male BALB/c (nu/nu) nude mice |
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Preparation Method |
Male BALB/c (nu/nu) nude mice, 5 weeks old and weighing 20g, were housed in a specific pathogen clearance grade (SPF) animal house at 24±2°C temperature and 55±5% humidity and maintained on a 12h light / 12h dark circadian rhythm. Mice received a standard diet and water AD libitum. U87MG cells (5×106 cells/100µl) were injected subcutaneously into the right abdomen of mice. When the tumor volume reached about 150mm3, the mice were randomly divided into two groups: the experimental group received D-Allose (100mg/kg; i.p.) once a day for 30 consecutive days; mice in the control group were intraperitoneally injected with normal saline. Tumor volume and body weight of mice were analyzed. |
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Dosage form |
100mg/kg/day for 30 days; i.p. |
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Applications |
D-Allose treatment suppressed tumor growth in the U87MG cell xenograft mouse models without affecting body weight. |
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References: |
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| Cas No. | 2595-97-3 | SDF | |
| Formula | C6H12O6 | M.Wt | 180.16 |
| Löslichkeit | DMSO : 100 mg/mL (555.06 mM; Need ultrasonic); H2O : 100 mg/mL (555.06 mM; Need ultrasonic) | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.5506 mL | 27.7531 mL | 55.5062 mL |
| 5 mM | 1.1101 mL | 5.5506 mL | 11.1012 mL |
| 10 mM | 555.1 μL | 2.7753 mL | 5.5506 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)