Deslanoside (Synonyms: Desacetyllanatoside C) |
Katalog-Nr.GC38903
One-Click Copy Product Info
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Deslanosid (Desacetyllanatosid C) ist ein schnell wirkendes Herzglykosid, das zur Behandlung von dekompensierter Herzinsuffizienz und supraventrikulÄren Arrhythmien aufgrund von Reentry-Mechanismen und zur Kontrolle der ventrikulÄren Frequenz bei der Behandlung von chronischem Vorhofflimmern verwendet wird.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17598-65-1
Sample solution is provided at 25 µL, 10mM.
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Deslanoside is a kind of steroid-like compounds extensively used to treat numerous heart conditions, such as cardiac arrhythmias and hypotension, by primarily inhibiting the Na+/K+-ATPase[1,2,3].
In vitro, Deslanoside inhibited cell viability in a dose-dependent manner, the 48-hour IC50 values were 8410nM, 370nM, and 180nM, for 22Rv1, PC-3, and DU 145, respectively, while 8.84μM for normal human prostatic epithelial cells (RWPE-1)[3]. The 48-hour IC50 values were 0.10μM, 0.05μM, 0.19μM, 0.07μM, and 0.12μM for A2780, A2780/CP, HCA46, HT29 and HCT116 respectively[4].
In vivo, treatment of 22Rv1 and PC-3 prostate cancer nude mouse subcutaneous tumor models with Deslanoside (5mg/kg; i.p.; 5 days per week; for 3 weeks), Deslanoside significantly inhibited tumor growth, the inhibition rates of 22Rv1 and PC-3 transplanted tumors were approximately 80% and 55%, respectively[3].
References:
[1]. Lingrel, Jerry B. "The physiological significance of the cardiotonic steroid/ouabain-binding site of the Na, K-ATPase." Annual review of physiology 72.1 (2010): 395-412.
[2]. Altamirano, Julio, et al. "The inotropic effect of cardioactive glycosides in ventricular myocytes requires Na+–Ca2+ exchanger function." The Journal of physiology 575.3 (2006): 845-854.
[3]. Liu M, Huang Q, A J, et al. The Cardiac Glycoside Deslanoside Exerts Anticancer Activity in Prostate Cancer Cells by Modulating Multiple Signaling Pathways. Cancers. 2021;13(22):5809.
[4]. Lauriola A, Uliassi E, Santucci M, et al. Identification of a Quinone Derivative as a YAP/TEAD Activity Modulator from a Repurposing Library. Pharmaceutics. 2022;14(2):391.
| Cell experiment [1]: | |
Cell lines | Human prostate cancer (PCa) cell lines 22Rv1, PC-3, DU 145, and normal human prostatic epithelial cells RWPE-1 |
Preparation Method | RWPE-1, PC-3, 22Rv1, and DU 145 cells were seeded onto 96-well plates at 6 × 103 cells per well, incubated overnight, and treated with various concentrations of Deslanoside (0, 0.002, 0.005, 0.014, 0.041, 0.123, 0.37, 1.11, 3.33, and 10μM) for 48h. Cell viability was measured using the Cell Counting Kit-8 (CCK-8) assay. |
Reaction Conditions | 0, 0.002, 0.005, 0.014, 0.041, 0.123, 0.37, 1.11, 3.33, and 10μM; 48h |
Applications | Deslanoside inhibited cell viability in a dose-dependent manner, the 48-hour IC50 values were 8410nM, 370nM, 180nM, and 8.84μM for 22Rv1, PC-3, DU 145, and RWPE-1 respectively. |
| Animal experiment [1]: | |
Animal models | Male Balb/c nude mice |
Preparation Method | Tumor cells of 22Rv1 (5 × 106 cells/site) or PC-3 cells (1 × 107 cells/site) in 200μL PBS mixed with high-concentration Matrigel (1:1) were subcutaneously injected into the right flank of mice. Tumor volumes were measured twice a week. When tumor volumes reached 150mm3 approximately, mice for each cell line were divided into two groups to receive the vehicle control (a solution of 5% DMSO, 30% PEG 300, 5% Tween-80, and 50% saline) and Deslanoside at 5mg/kg/bodyweight via i.p. (5 days per week). During deslanoside administration, mouse body weights and tumor volumes were measured twice a week. |
Dosage form | 5mg/kg/bw; i.p.; 5 days per week, for 3 weeks |
Applications | Deslanoside significantly inhibits tumor growth. The inhibition rates of 22Rv1 and PC-3 transplanted tumors are approximately 80% and 55%, respectively. |
References: | |
| Cas No. | 17598-65-1 | SDF | |
| Überlieferungen | Desacetyllanatoside C | ||
| Formula | C47H74O19 | M.Wt | 943.08 |
| Löslichkeit | DMSO: 100 mg/mL (106.04 mM) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0604 mL | 5.3018 mL | 10.6036 mL |
| 5 mM | 212.1 μL | 1.0604 mL | 2.1207 mL |
| 10 mM | 106 μL | 530.2 μL | 1.0604 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)