Z-Gly-Pro-pNA (Synonyms: Carbobenzoxy-glycyl-L-prolyl-p-Nitroanilide, N-CBZ-Glycyl-L-proline 4-Nitroanilide, Z-Gly-Pro-4-Nitroanilide)

Gly-Pro-pNA, as a chromogenic substrate, can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV).^[1]

In vitro, when Z-Gly-Pro-pNA was used as a substrate, SNA-8073-B inhibited prolyl endopeptidase of Flavobacterium non-competitively with IC50 of 8.9 µM.^[2] In addition, when Z-Gly-Pro-pNA was used as a substrate, Propeptin inhibited prolyl endopeptidase of the genus Flavobacterium competitively with Ki of 0.70 µM.^[3] In addition, using Gly-Pro-p-nitroanilide as substrate to determine the enzymatic activity of this molecule in serum. Using Gly-Pro-pNA as substrate can assess the enzymatic activity and biochemical status of dipeptidyl peptidase IV in patients with rheumatoid arthritis.^[4] In vitro, using Gly-Pro-pNA as a substrate can determine DPP-IV inhibitory activities of quinoa protein hydrolysates. Twenty quinoa-derived peptides were determined with in vitro DPP-IV inhibitory activities with IC50 values less than 500 µM. ^[5]

^[1] Fu P, et al. Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. Gene. 2018 Aug 15;667:101-111.

Kimura K, et al. SNA-8073-B, a new isotetracenone antibiotic inhibits prolyl endopeptidase. I. Fermentation, isolation and biological properties. J Antibiot (Tokyo). 1997 Apr;50(4):291-6.

Kimura K, et al. Propeptin, a new inhibitor of prolyl endopeptidase produced by Microbispora. I. Fermentation, isolation and biological properties. J Antibiot (Tokyo). 1997 May;50(5):373-8.

Yeganeh F, et al. Association of CD26/dipeptidyl peptidase IV mRNA level in peripheral blood mononuclear cells with disease activity and bone erosion in rheumatoid arthritis. Clin Rheumatol. 2018 Dec;37(12):3183-3190.

You H, et al. Preparation and identification of dipeptidyl peptidase IV inhibitory peptides from quinoa protein. Food Res Int. 2022 Jun;156:111176.

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