IPTG (Isopropyl β-D-thiogalactoside) (Synonyms: Isopropyl βthiogalactoside) |
| Katalog-Nr.GC30002 |
IPTG (Isopropyl β-D-Thiogalactosid) ist eine molekulare Nachahmung von Allolactose, einem Lactosemetaboliten, der die Transkription des lac-Operons auslÖst, und wird daher verwendet, um die Proteinexpression zu induzieren, wobei das Gen unter der Kontrolle des lac-Operators steht.
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Cas No.: 367-93-1
Sample solution is provided at 25 µL, 10mM.
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease[1].
[1]. Isopropyl β-D-1-thiogalactopyranoside
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