1,5-Isoquinolinediol (Synonyms: NSC 65585) |
| カタログ番号GC40468 |
1,5-イソキノリンジオールは、IC50 が 0.18 ～ 0.37 μM の強力な PARP 阻害剤です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5154-02-9
Sample solution is provided at 25 µL, 10mM.
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The poly(ADP-ribose) polymerases (PARPs) form a family of enzymes with roles in DNA repair and apoptosis. [1] 1,5-Isoquinolinediol is an inhibitor of poly(ADP-ribose) synthetase (PARP1; IC50 = 0.39 μM). [2] It has been used to study the role of PARP1 in both DNA repair and oxidant stress-induced cell death. [3][4][5] This compound can be used with cells in culture and in animals.[4][6][7]
Reference:
[1]. Davar, D., Beumer, J.H., Hamieh, L., et al. Role of PARP inhibitors in cancer biology and therapy. Current Medicinal Chemistry 19(23), 3907-3921 (2012).
[2]. Banasik, M., Komura, H., Shimoyama, M., et al. Specific inhibitors of poly(ADP-ribose) synthetase and mono(ADP-ribosyl)transferase. The Journal of Biological Chemisty 267(3), 1569-1575 (1992).
[3]. Ruscetti, T., Lehnert, B.E., Halbrook, J., et al. Stimulation of the DNA-dependent protein kinase by poly(ADP-ribose) polymerase. The Journal of Biological Chemisty 273(23), 14461-14467 (1998).
[4]. Chatterjee, P.K., Zacharowski, K., Cuzzocrea, S., et al. Inhibitors of poly (ADP-ribose) synthetase reduce renal ischemia-reperfusion injury in the anesthetized rat in vivo. The FASEB Journal 14(5), 641-651 (2000).
[5]. Bowes, J., McDonald, M.C., Piper, J., et al. Inhibitors of poly (ADP-ribose) synthetase protect rat cardiomyocytes against oxidant stress. Cardiovascular Research 41(1), 126-134 (1999).
[6]. Byun, J.Y., Kim, M.J., Eum, D.Y., et al. Reactive oxygen species-dependent activation of Bax and poly(ADP-ribose) polymerase-1 is required for mitochondrial cell death induced by triterpenoid pristimerin in human cervical cancer cells. Molecular Pharmacology 76(4), 734-744 (2009).
[7]. Kang, Y.H., Yi, M.J., Kim, M.J., et al. Caspase-independent cell death by arsenic trioxide in human cervical cancer cells: Reactive oxygen species-mediated poly(ADP-ribose) polymerase-1 activation signals apoptosis-inducing factor release from mitochondria. Cancer Research 64(24), 8960-8967 (2004).
| Cas No. | 5154-02-9 | SDF | |
| 同義語 | NSC 65585 | ||
| Chemical Name | 5-hydroxy-1(2H)-isoquinolinone | ||
| Canonical SMILES | OC1=C2C(C(NC=C2)=O)=CC=C1 | ||
| Formula | C9H7NO2 | M.Wt | 161.2 |
| 溶解度 | DMSO : 37.04 mg/mL (229.83 mM; Need ultrasonic) | Storage | Store at 4°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 6.2035 mL | 31.0174 mL | 62.0347 mL |
| 5 mM | 1.2407 mL | 6.2035 mL | 12.4069 mL |
| 10 mM | 0.6203 mL | 3.1017 mL | 6.2035 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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