Ani 9 |
カタログ番号GC46086
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Ani 9は高い選択性のANO1の阻害剤であり、ANO1チャネルの活性を有力に遮断し、IC₅₀は3μM以下である。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 356102-14-2
Sample solution is provided at 25 µL, 10mM.
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Ani 9は高い選択性のANO1の阻害剤であり、ANO1チャネルの活性を有力に遮断し、IC₅₀は3μM以下である。Ani 9は細胞内カルシウムシグナル伝達とCFTR塩化物チャネルの活性を影響しなかった。Ani 9は、ANO1を研究する有用な薬理学的ツールであり、癌、高血圧、疼痛、下痢と喘息の薬物治療の潜在的な開発候補となる可能性がある[1]。
体外で、3-30μM Ani 9の24-72時間の処理は、PC-3細胞の成長を用量依存的と時間依存的に阻害した[2]。最大30μMのAni 9で20分間処理した結果、FRT細胞でATP誘発の細胞質カルシウムの増加を影響しなかった[1]。1μM、3μMと10μMのAni 9の20分間の処理は、LN215でVRACの活性をそれぞれ13.5±1.1%、21.4±1.0%と40.7±1.5%阻害した[1]。3μM Ani9の30分間の処理は、雄のマウスUSMの電界刺激（EFS）誘発収縮を影響しなかった[3]。10μM Ani 9の30分間の処理は、カルバコールで誘発されたマウスの気管支拘縮に、遮断効果を持たなかった[4]。
References:
[1] Seo Y, Lee HK, Park J, et al. Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PLoS One. 2016;11(5):e0155771.
[2] Song Y, Gao J, Guan L, Chen X, Gao J, Wang K. Inhibition of ANO1/TMEM16A induces apoptosis in human prostate carcinoma cells by activating TNF-α signaling. Cell Death Dis. 2018;9(6):703.
[3] Gupta N, Baker SA, Sanders KM, et al. ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions. Sci Rep. 2025;15(1):17365.
[4] Dwivedi R, Drumm BT, Alkawadri T, et al. The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca2+-release in mouse bronchial smooth muscle cells. Eur J Pharmacol.
| 細胞実験[1]: | |
細胞株 | PC-3細胞 |
準備方法 | PC-3細胞を様々な濃度のAni9で24-72時間処理した。細胞の増殖をCCK-8アッセイで測定した。 |
反応条件 | 3-30μM；24-72時間 |
アプリケーション | Ani 9は細胞の成長を用量と時間依存的に阻害した。 |
References: | |
| Cas No. | 356102-14-2 | SDF | |
| Chemical Name | 2-(4-chloro-2-methylphenoxy)-acetic acid, 2-[(2-methoxyphenyl)methylene]hydrazide | ||
| Canonical SMILES | ClC1=CC=C(OCC(N/N=C/C2=CC=CC=C2OC)=O)C(C)=C1 | ||
| Formula | C17H17ClN2O3 | M.Wt | 332.8 |
| 溶解度 | 25mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0048 mL | 15.024 mL | 30.0481 mL |
| 5 mM | 601 μL | 3.0048 mL | 6.0096 mL |
| 10 mM | 300.5 μL | 1.5024 mL | 3.0048 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)