Ani 9 |
Katalog-Nr.GC46086
One-Click Copy Product Info
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Ani 9 ist ein hochselektiver Inhibitor von ANO1. Es blockiert wirksam die ANO1-Kanalsktivität mit einem IC₅₀-Wert unter 3 μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 356102-14-2
Sample solution is provided at 25 µL, 10mM.
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Ani 9 ist ein hochselektiver Inhibitor von ANO1. Es blockiert wirksam die ANO1-Kanalsktivität mit einem IC₅₀-Wert unter 3 μM. Ani 9 beeinflusst weder den intrazellulären Calcium-Signalweg noch die Aktivität des CFTR-Chloridkanals. Ani 9 kann ein nützliches pharmakologisches Werkzeug zur Untersuchung von ANO1 sein. Und es kann ein potentieller Kandidat für die Entwicklung von Arzneimitteln gegen Krebs, Hypertonie, Schmerz, Diarrhoe und Asthma sein[1].
In vitro hemmte die Behandlung mit 3-30 μM Ani 9 über 24-72 Stunden das Wachstum von PC-3-Zellen in dosis- und zeitabhängiger Weise[2]. Die Anwendung von Ani 9 bis 30 μM über 20 Minuten beeinflusste den ATP-induzierten Anstieg des zytosolischen Calciums in FRT-Zellen nicht. 1 μM, 3 μM und 10 μM Ani hemmten die VRAC-Aktivität in LN215-Zellen über 20 Minuten um 13.5±1.1%, 21.4±1.0% bzw. 40.7±1.5%[1]. 3 μM Ani9 über 30 Minuten beeinflusste die durch elektrische Feldstimulation(EFS) ausgelösten Kontraktionen des männlichen murinen USM nicht[3]. 10μM Ani 9 über 30 Minuten hatte keine blockierende Wirkung auf die durch Carbachol induzierten Kontraktionen von Maus-Bronchien[4].
References:
[1] Seo Y, Lee HK, Park J, et al. Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PLoS One. 2016;11(5):e0155771.
[2] Song Y, Gao J, Guan L, Chen X, Gao J, Wang K. Inhibition of ANO1/TMEM16A induces apoptosis in human prostate carcinoma cells by activating TNF-α signaling. Cell Death Dis. 2018;9(6):703.
[3] Gupta N, Baker SA, Sanders KM, et al. ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions. Sci Rep. 2025;15(1):17365.
[4] Dwivedi R, Drumm BT, Alkawadri T, et al. The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca2+-release in mouse bronchial smooth muscle cells. Eur J Pharmacol.
| Zellexperiment [1]: | |
Zelllinien | PC-3-Zelle |
Vorbereitungsmethode | PC-3-Zellen wurden 24-72 Stunden lang mit verschiedenen Konzentrationen von Ani 9 behandelt. Die Zellproliferation wurde mittels CCK-8-Assay gemessen. |
Reaktionsbedingungen | 3-30 μM; 24-72 Stunden |
Anwendungen | Ani 9 hemmte das Zellwachstum dosis- und zeitabhängig. |
References: | |
| Cas No. | 356102-14-2 | SDF | |
| Chemical Name | 2-(4-chloro-2-methylphenoxy)-acetic acid, 2-[(2-methoxyphenyl)methylene]hydrazide | ||
| Canonical SMILES | ClC1=CC=C(OCC(N/N=C/C2=CC=CC=C2OC)=O)C(C)=C1 | ||
| Formula | C17H17ClN2O3 | M.Wt | 332.8 |
| Löslichkeit | 25mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0048 mL | 15.024 mL | 30.0481 mL |
| 5 mM | 601 μL | 3.0048 mL | 6.0096 mL |
| 10 mM | 300.5 μL | 1.5024 mL | 3.0048 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)