Ani 9 |
Catalog No.GC46086
One-Click Copy Product Info
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Ani 9 is a highly selective inhibitor of ANO1 that potently blocks ANO1 channel activity with an IC₅₀ below 3μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 356102-14-2
Sample solution is provided at 25 µL, 10mM.
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Ani 9 is a highly selective inhibitor of ANO1 that potently blocks ANO1 channel activity with an IC₅₀ below 3μM. Ani 9 did not affect the intracellular calcium signaling and CFTR chloride channel activity. Ani 9 may be a useful pharmacological tool for studying ANO1 and a potential development candidate for drug therapy of cancer, hypertension, pain, diarrhea and asthma[1].
In vitro, treatment with 3-30μM Ani 9 for 24-72h inhibited PC-3 cell growth in dose-dependent and time-dependent manner[2]. Application of Ani 9 up to 30μM for 20min did not affect the ATP-induced cytosolic calcium increase in FRT cells[1]. 1μM, 3μM and 10μM Ani 9 for 20min inhibited VRAC activity by 13.5±1.1%, 21.4±1.0% and 40.7±1.5%, respectively in LN215[1]. 3μM Ani9 for 30min did not affect electrical field stimulation (EFS)-evoked contractions of male murine USM[3]. 10μM Ani 9 for 30min had no blocking effect on contractures of mouse bronchi induced by carbachol[4].
References:
[1] Seo Y, Lee HK, Park J, et al. Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PLoS One. 2016;11(5):e0155771.
[2] Song Y, Gao J, Guan L, Chen X, Gao J, Wang K. Inhibition of ANO1/TMEM16A induces apoptosis in human prostate carcinoma cells by activating TNF-α signaling. Cell Death Dis. 2018;9(6):703.
[3] Gupta N, Baker SA, Sanders KM, et al. ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions. Sci Rep. 2025;15(1):17365.
[4] Dwivedi R, Drumm BT, Alkawadri T, et al. The TMEM16A blockers benzbromarone and MONNA cause intracellular Ca2+-release in mouse bronchial smooth muscle cells. Eur J Pharmacol.
| Cell experiment [1]: | |
Cell lines | PC-3 cell |
Preparation Method | PC-3 cells were treated with different concentrations of Ani9 for 24-72h. Cell proliferation was measured by CCK-8 assay. |
Reaction Conditions | 3-30μM; 24-72h |
Applications | Ani 9 inhibited cell growth in dose-dependent and time-dependent manner. |
References: | |
| Cas No. | 356102-14-2 | SDF | |
| Chemical Name | 2-(4-chloro-2-methylphenoxy)-acetic acid, 2-[(2-methoxyphenyl)methylene]hydrazide | ||
| Canonical SMILES | ClC1=CC=C(OCC(N/N=C/C2=CC=CC=C2OC)=O)C(C)=C1 | ||
| Formula | C17H17ClN2O3 | M.Wt | 332.8 |
| Solubility | 25mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0048 mL | 15.024 mL | 30.0481 mL |
| 5 mM | 601 μL | 3.0048 mL | 6.0096 mL |
| 10 mM | 300.5 μL | 1.5024 mL | 3.0048 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)