CORM-3 |
カタログ番号GC18071
One-Click Copy Product Info
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CORM-3は水溶性のCO放出分子で、リン酸担体との相互作用によりミトコンドリア呼吸のカップリングを解除する。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 475473-26-8
Sample solution is provided at 25 µL, 10mM.
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CORM-3は水溶性のCO放出分子で、リン酸担体との相互作用によりミトコンドリア呼吸のカップリングを解除する。NF-κB p65の核異所性を低減することができ、活性酸素の生成を抑え、細胞内のグルタチオンとスーパーオキシドディスムターゼのレベルを高める。さらに、CORM-3はNLRP3インフラマソームの活性化を抑制する[1-3]。
CORM-3(0-400μM；2時間)は用量依存的にH2O2がBMSCの生存率に及ぼす影響を阻害した。CORM-3は、活性酸素種（ROS）の蓄積を抑制し、ミトコンドリアの機能不全を予防することで、過酸化水素（H2O2）曝露によって破壊されたBMSCsの骨形成能を回復させることができる[4]。CORM-3(20μM ;30分)はDNAにRu(II)が結合することでDNA損傷を引き起こす[5]。
CORM-3（4mg/kg；i.v.）はHSR誘発腸管障害を軽減した[6]。CORM-3はミクログリア（MG）の活性化によって引き起こされる炎症反応を効果的に改善することができ、神経細胞のアポトーシスを抑え、神経再生を促進し、放射線被曝後のマウスの学習・記憶能力を改善した[7]。
References:
[1]. Long R, Salouage I, Berdeaux A, et,al. CORM-3, a water soluble CO-releasing molecule, uncouples mitochondrial respiration via interaction with the phosphate carrier. Biochim Biophys Acta. 2014 Jan;1837(1):201-9. doi: 10.1016/j.bbabio.2013.10.002. Epub 2013 Oct 23. PMID: 24161358.
[2]. Huang Y, Ma T, et,al. Carbon monoxide (CO) inhibits hydrogen peroxide (H2O2)-induced oxidative stress and the activation of NF-κB signaling in lens epithelial cells. Exp Eye Res. 2018 Jan;166:29-39. doi: 10.1016/j.exer.2017.08.016. Epub 2017 Oct 16. PMID: 29051011.
[3]. Lee DW, Shin HY, et,al. Carbon monoxide regulates glycolysis-dependent NLRP3 inflammasome activation in macrophages. Biochem Biophys Res Commun. 2017 Nov 18;493(2):957-963. doi: 10.1016/j.bbrc.2017.09.111. Epub 2017 Sep 21. PMID: 28942141.
[4]. Jin C, Lin BH, et,al. CORM-3 Attenuates Oxidative Stress-Induced Bone Loss via the Nrf2/HO-1 Pathway. Oxid Med Cell Longev. 2022 Aug 17;2022:5098358. doi: 10.1155/2022/5098358. PMID: 36035220; PMCID: PMC9402314.
[5]. Lyon RF, Southam HM, et,al. CORM-3 induces DNA damage through Ru(II) binding to DNA. Biochem J. 2022 Jul 15;479(13):1429-1439. doi: 10.1042/BCJ20220254. PMID: 35726678; PMCID: PMC9342897.
[6]. Zhang LM, Xin Y, et,al. CORM-3 alleviates the intestinal injury in a rodent model of hemorrhage shock and resuscitation: roles of GFAP-positive glia. J Mol Histol. 2023 Aug;54(4):271-282. doi: 10.1007/s10735-023-10133-w. Epub 2023 Jun 19. PMID: 37335421.
[7]. Lu K, Wu WJ, et,al. CORM-3 Regulates Microglia Activity, Prevents Neuronal Injury, and Improves Memory Function During Radiation-induced Brain Injury. Curr Neurovasc Res. 2020;17(4):464-470. doi: 10.2174/1567202617999200730213259. PMID: 32748746.
| 細胞実験[1]: | |
細胞株 | BMSCs(Sprague-Dawleyラット) |
準備方法 | 細胞を96ウェルプレートに密度で接種し、H2O2処理の有無にかかわらず、さまざまなCORM-3濃度(0、12.5、25、50、100、200、および400μM)で2時間処理した。 |
反応条件 | 0、12.5、25、50、100、200、および400μM;2時間 |
アプリケーション | CORM-3は、H2O2がBMSCsの生存率に及ぼす影響を用量依存的に阻害した。 |
| 動物実験 [2]: | |
動物モデル | 雄SDラット(出血性ショックおよび蘇生法(HSR)モデル) |
準備方法 | HSRラットにCORM-3を静脈内投与した。HSRモデリングの24時間後と7日後に、腸組織の病理学的変化が評価された。 |
投与形態 | 4 mg / kg; i.v |
アプリケーション | CORM-3はHSRによる腸の損傷を軽減した。 |
参考文献: | |
| Cas No. | 475473-26-8 | SDF | |
| Canonical SMILES | O=C1[O-][Ru+2]([Cl-])([C]=O)([C]=O)([C]=O)[NH2]C1 | ||
| Formula | C5H4ClNO5Ru | M.Wt | 294.61 |
| 溶解度 | DMSO : 100 mg/mL; Water : 50 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3943 mL | 16.9716 mL | 33.9432 mL |
| 5 mM | 678.9 μL | 3.3943 mL | 6.7886 mL |
| 10 mM | 339.4 μL | 1.6972 mL | 3.3943 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)