LDAO (Synonyms: Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide) |
カタログ番号GC44046
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LDAO (Lauryl Dimethylamine Oxide)は非イオン性/両性イオン性の洗剤であり、膜タンパク質の溶解と安定化に使われることができる。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1643-20-5
Sample solution is provided at 25 µL, 10mM.
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LDAO (Lauryl Dimethylamine Oxide)は非イオン性/両性イオン性の洗剤であり、膜タンパク質の溶解と安定化に使われることができる。LDAOのマイルドな浄化能力はタンパク質の天然形態を維持すると同時に、脂質二重層の構造を効果的に破壊させる[2]。LDAOは細菌または哺乳類細胞膜における膜タンパク質（例えばGタンパク質共役受容体など）の溶解に使われ、その同時に、機能活性を維持する[3]。LDAOはミトコンドリア膜構造を溶解することで、H+-ATPaseの活性を間接的に影響することができ、よってATP加水分解プロセスを調節する[4]。LDAOを無銅クリック化学反応に添加した結果、CRM197変異体の肺炎球菌多糖体への結合効率を著しく増やした[5]。LDAOをRhodovulum sulfidophilumの成長培地に添加した結果、カロテノイド生合成を劇的に変化させた[6]。
References:
[1] Veldhuis G, Gabellieri E, Poolman B, et al. The effect of the detergent-shell on the structure of a membrane-protein[J]. Mechanics of the mannitol transporter from Escherichia coli, 111.
[2] Orwick‐Rydmark M, Arnold T, Linke D. The use of detergents to purify membrane proteins[J]. Current protocols in protein science, 2016, 84(1): 4.8. 1-4.8. 35.
[3] Marcovich I. Structural studies of enzymes: a bacterial flippase and a human kinase[J]. 2020.
[4] Vazquez-Laslop N, Dreyfus G. Mitochondrial H+-ATPase activation by an amine oxide detergent[J]. Journal of Biological Chemistry, 1986, 261(17): 7807-7810.
[5] Bautista L, Pill-Pepe L, Kapoor N, et al. Addition of lauryldimethylamine N-oxide (LDAO) to a copper-free click chemistry reaction improves the conjugation efficiency of a cell-free generated CRM197 variant to clinically important Streptococcus pneumoniae serotypes[J]. ACS omega, 2022, 7(39): 34921-34928.
[6] Hagemann G E, Sturgis J N, Wagner E, et al. The effects of the detergent LDAO on the carotenoid metabolism and growth of Rhodovulum sulfidophilum[J]. Microbiological research, 1996, 151(4): 421-426.
| Cas No. | 1643-20-5 | SDF | |
| 同義語 | Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide | ||
| Canonical SMILES | CCCCCCCCCCCC[N+](C)([O-])C | ||
| Formula | C14H31NO | M.Wt | 229.4 |
| 溶解度 | DMF: 0.3 mg/ml,DMSO: 0.1 mg/ml,Ethanol: 15 mg/ml,PBS (pH 7.2): 0.1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.3592 mL | 21.796 mL | 43.592 mL |
| 5 mM | 871.8 μL | 4.3592 mL | 8.7184 mL |
| 10 mM | 435.9 μL | 2.1796 mL | 4.3592 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)