Fisetin-d5

Fisetin-d₅ is intended for use as an internal standard for the quantification of fisetin by GC- or LC-MS. Fisetin is a flavonoid that has been found in various fruits and vegetables and has diverse biological activities.^1,2,3,4 It scavenges radicals in a Trolox equivalent antioxidant capacity (TEAC) assay and inhibits oxidative burst induced by N-Formyl-Met-Leu-Phe in isolated human polymorphonuclear neutrophils (PMNs) when used at a concentration of 10 µM.^1,3 Fisetin (10-60 µM) induces cell cycle arrest at the G₁ phase and apoptosis in, as well as inhibits the proliferation of, LNCaP prostate cancer cells.² It inhibits LPS-induced production of nitrite, prostaglandin E₂ , and NF-κB activation in RAW 264.7 cells.³ Fisetin (1 mg/animal) reduces tumor growth in a CWR22RΥ1 prostate cancer mouse xenograft model.⁴

1.Khan, N., Syed, D.N., Ahmad, N., et al.Fisetin: A dietary antioxidant for health promotionAntioxid. Redox Signal.19(2)151-162(2013) 2.Khan, N., Afaq, F., Syed, D.N., et al.Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cellsCarcinogenesis29(5)1049-1056(2008) 3.Wang, L., Tu, Y.-C., Lian, T.-W., et al.Distinctive antioxidant and antiinflammatory effects of flavonolsJ. Agric. Food Chem.54(26)9798-9804(2006) 4.Khan, N., Asim, M., Afaq, F., et al.A novel dietary flavonoid fisetin inhibits androgen receptor signaling and tumor growth in athymic nude miceCancer Res.68(20)8555-8563(2008)