Mitomycin C (Synonyms: Ametycine) |
Katalog-Nr.GC12353 |
Mitomycin C, eine Art von Antibiotikum, das aus Streptomyces caespitosus oder Streptomyces lavendulae isoliert wurde, hemmt die DNA-Synthese durch kovalente Mitomycin-C-DNA-Addukte mit EC50-Werten von 0,14 μM in PC3-Zellen.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1950/7/7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1, 2]: | |
Cell lines |
HCT116, HT-29 |
Preparation Method |
Ten millimolar Mitomycin C is prepared in 100% dimethyl sulfoxide, stored as small aliquots at -80°C and then diluted as needed in cell culture medium. |
Reaction Conditions |
5 μM,12 or 24h |
Applications |
Mitomycin C is a mitomycin that is used as a chemotherapeutic agent by virtue of its antitumour activity. Mitomycin C not only potentiates TRAIL-induced apoptosis in HCT116 (p53−/−) colon cancer cells but also sensitizes TRAIL- resistant colon cancer cells HT-29 to the cytokine. Mitomycin C inhibits HT-29 with IC50 of 40 nM. |
Animal experiment [1]: | |
Animal models |
Nude mice (6 weeks) injected subcutaneously with 1 × 106 HCT116 (p53−/−) or 2 × 106 HT-29 cells mixed with Matrigel |
Preparation Method |
Ten millimolar Mitomycin C is prepared in 100% dimethyl sulfoxide, stored as small aliquots at -80°C and then diluted as needed in cell culture medium. |
Dosage form |
1 mg/kg, Intraperitoneal injection |
Applications |
Mitomycin C suppresses tumor growth significantly and does not impact the weight of the mice with TRAIL, indicating that the therapeutic combination of Mitomycin C and TRAIL is well-tolerated and has anti-tumor activity in vivo. |
References: [1]. Cheng H, et al. Mitomycin C potentiates TRAIL-induced apoptosis through p53-independent upregulation of death receptors: evidence for the role of c-Jun N-terminal kinase activation. Cell Cycle. 2012 Sep 1;11(17):3312-23. [2]. Hodgkinson TJ, et al. Chemical synthesis and cytotoxicity of some azinomycin analogues devoid of the 1-azabicyclo[3.1.0]hexane subunit. Bioorg Med Chem Lett. 2000 Feb 7;10(3):239-41. |
Mitomycin C, eine Art von Antibiotikum, das aus Streptomyces caespitosus oder Streptomyces lavendulae isoliert wurde, hemmt die DNA-Synthese durch kovalente Mitomycin-C-DNA-Addukte mit EC50-Werten von 0,14 μM in PC3-Zellen.
Mitomycin C ist ein Antibiotikum, das in präklinischen und klinischen Studien eine antitumorale Aktivität gezeigt hat und weit verbreitet zur Behandlung verschiedener Krebsarten eingesetzt wird. Mitomycin C wirkt synergistisch mit Capecitabin und Irinotecan. Einige Studien legen nahe, dass die Kombination von 5-FU plus Mitomycin C in vitro bei kolorektalem Krebs aktiver ist als die Monotherapie. Die Wirksamkeit der Kombination von Mitomycin C mit anderen zytotoxischen Wirkstoffen wie Capecitabin und Raltiterxed bei kolorektalem Krebs wurde berichtet.[1]
Mitomycin C potenziert nicht nur die TRAIL-induzierte Apoptose in HCT116 (p53−/−) Darmkrebszellen, sondern sensibilisiert auch TRAIL-resistente Darmkrebszellen HT-29 für das Zytokin. Auf mechanischer Ebene reguliert Mitomycin C Überlebensproteine wie Bcl2, Mcl-1 und Bcl-XL herunter und regt pro-apoptotische Proteine wie Bax, Bim und die Oberflächenexpression von TRAIL-Todesrezeptoren DR4 und DR5 an. Außerdem zeigt das Ergebnis des Zellversuchs, dass Mitomycin C HT-29 mit einer IC50 von 40 nM hemmt. [1,2]
Mitomycin C spielt auch eine wirksame Rolle bei der Antitumortherapie in vivo. Für das In-vivo-Experiment unterdrückte Mitomycin C das Tumorwachstum signifikant und hatte keinen Einfluss auf das Gewicht der Mäuse mit TRAIL, was darauf hinweist, dass die therapeutische Kombination aus Mitomycin C und TRAIL gut verträglich ist und in vivo eine antitumorale Aktivität aufweist. [1]
Cas No. | 1950/7/7 | SDF | |
Überlieferungen | Ametycine | ||
Chemical Name | ((1aS,8S,8aR,8bS)-6-amino-8a-methoxy-5-methyl-4,7-dioxo-1,1a,2,4,7,8,8a,8b-octahydroazirino[2',3':3,4]pyrrolo[1,2-a]indol-8-yl)methyl carbamate | ||
Canonical SMILES | NC(C1=O)=C(C)C(C2=C1[C@@H](COC(N)=O)[C@]3(OC)N2C[C@H]4[C@@H]3N4)=O | ||
Formula | C15H18N4O5 | M.Wt | 334.33 |
Löslichkeit | ≥ 16.7mg/mL in DMSO | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.9911 mL | 14.9553 mL | 29.9106 mL |
5 mM | 0.5982 mL | 2.9911 mL | 5.9821 mL |
10 mM | 0.2991 mL | 1.4955 mL | 2.9911 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *