Epibrassinolide (Synonyms: EBL, 24-Epibrassinolide) |
| カタログ番号GC11834 |
Epibrassinolide (24-Epibrassinolide) は、どこにでも存在する植物成長ホルモンであり、植物の重金属や農薬ストレスを軽減する大きな可能性を示しています。エピブラシノリドは、非腫瘍細胞の増殖に影響を与えることなく、さまざまな癌細胞の潜在的なアポトーシス誘導因子です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 78821-43-9
Sample solution is provided at 25 µL, 10mM.
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Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants[1]. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth[2].
Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells[1].
References:
[1]. Shahzad B, et al. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotoxicol Environ Saf. 2018 Jan;147:935-944.
[2]. Obakan P, et al. Activation of polyamine catabolic enzymes involved in diverse responses against epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines.Amino Acids. 2014 Mar;46(3):553-64.
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Cell experiment: |
LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO2 incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×104 density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically[1]. |
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References: [1]. Shahzad B, et al. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotoxicol Environ Saf. 2018 Jan;147:935-944. |
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| Cas No. | 78821-43-9 | SDF | |
| 同義語 | EBL, 24-Epibrassinolide | ||
| Chemical Name | (3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-((2S,3R,4R,5R)-3,4-dihydroxy-5,6-dimethylheptan-2-yl)-5,6-dihydroxy-7a,9a-dimethyltetradecahydro-1H-benzo[c]indeno[5,4-e]oxepin-3(12bH)-one | ||
| Canonical SMILES | CC([C@@]([C@](O)([H])[C@@](O)([H])[C@@]([C@@]1([H])CC[C@@]2([H])[C@]([C@]3([H])CC[C@]12C)([H])COC([C@@]4([H])C[C@@](O)([H])[C@@](O)([H])C[C@]34C)=O)([H])C)([H])C)C | ||
| Formula | C28H48O6 | M.Wt | 480.68 |
| 溶解度 | DMF: 5 mg/mL,DMF:PBS (pH 7.2)(1:3): 0.25 mg/mL,DMSO: 3 mg/mL,Ethanol: 3 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0804 mL | 10.4019 mL | 20.8039 mL |
| 5 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL |
| 10 mM | 0.208 mL | 1.0402 mL | 2.0804 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)
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