RSL3
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カタログ番号GC12431
One-Click Copy Product Info
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RSL3はGPX4阻害剤の一種のであり、脂質の過酸化を惹起し、GPX4の発現を低下させる。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1219810-16-8
Sample solution is provided at 25 µL, 10mM.
- Clin. Transl. Med. 15.3 (2025):e70270.PMID:40088428
- Int J Mol Sci 22.21 (2021):11334.PMID:34768761
- J Agr Food Chem (2025).PMID:40493028
- Chem-Biol Interact (2023):110828.PMID:38081571
- Eur J Pharmacol (2026):178864.
- Neuropharmacology 298 (2026):111050.PMID:42214457
- Bba-Mol Basis Dis: (2026): 168432.PMID:42637154
- Neurochem Res 50.3 (2025):149.PMID:40257585
- Diabetol Metab Syndr (2026).PMID:41530851
- Pathogens 14.6 (2025):590.
- Mol Oral Microbiol: (2026).PMID:42713739
- Available at SSRN 4854749.
- Int immunopharmacol 141 (2024) 113020:
RSL3はGPX4阻害剤の一種のであり、脂質の過酸化を惹起し、GPX4の発現を低下させる[1]。RSL3はグリオーマ細胞で、酸化ストレスを誘発し、グルタチオンS-トランスフェラーゼP1（GSTP1）を上方制御し、TGM2のS-グルタチオン化を促進し、最終的にはTGM2のプロテアソーム分解を起こし、よってグリオーマ細胞は電離放射線により敏感的になる[2]。RSL3は、様々な癌細胞で、フェロトーシスとアポトーシスの誘発に日常的に使われている[3]。
In vitro（体外）実験で、RSL3で48時間処理したところ、4T1とMCF-7細胞の生存率が著しく阻害され、IC50値は3.69μMと13.57μMであった[4]。80nM RSL3で24時間処理したところ、MDA-MB-231細胞で細胞死が著しく誘発され、ミトコンドリア膜電位が低下した[5]。5μM RSL3で12時間処理したところ、LoVo細胞で、PARP1切断が促進され、DNA損傷が誘発され、同時に細胞の増殖が減少した[6]。
In vivo（体内）実験で、10mg/kgのRSL3を15日間毎日腹腔内注射したところ、ヌードマウスで、グリオーマ細胞の増殖が著しく阻害され、解糖機能障害が誘発された[7]。100mg/kgのRSL3を2週間、2週間ごとに腫瘍内注射したところ、U87異種移植マウスモデルで、腫瘍の増殖が著しく阻害された[8]。
| 細胞実験 [1]: | |
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細胞株 |
4T1細胞 |
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調製方法 |
37°C、5% CO2の環境で、10%のウシ胎児血清（FBS）、1%のペニシリン-ストレプトマイシンを添加したDMEM培地で4T1細胞を培養した。4T1細胞を96ウェルのマイクロプレート（5×103個の細胞/ウェル）に播種して、24時間培養した。10%のFBSの存在下で、濃度が増加する（0、0.01、0.1、1、10と100μM）RSL3で細胞を48時間処理し、細胞の生存率を評価した。 |
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反応条件 |
0、0.01、0.1、1、10と100μM；48時間 |
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応用 |
RSL3の処理により、4T1細胞の生存率は用量依存的に低下した。 |
| 動物実験 [2]: | |
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動物モデル |
無胸腺BALB/cヌードマウス |
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調製方法 |
温度（21-23°C）、湿度（30-35%）と明暗周期（午前7時から午後7時まで点灯）がコントロールされた環境で、無胸腺BALB/cヌードマウス（4週齢；20-22g）を飼育し、標準齧歯類飼料を投与した。100μlのPBSの中の計1×106個の対数増殖期のC6細胞を各マウスの右脇腹に皮下注射した。7日後、腫瘍の体積が約150mm3に達した時に、RSL3処理を開始した。マウスを分け、それぞれ15日間、毎日一回、溶媒（対照群、n=10/群）または10mg/kg RSL3（n=10/群）を50μl腹腔内注射した。腫瘍の体積を式（0.5×A×B2、Aは腫瘍の長さ、Bはその幅を意味する）で算出した。 |
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剤形 |
10mg/kg/日；15日間；腹腔内注射 |
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応用 |
RSL3の処理により、C6異種移植片を持つマウスで、腫瘍の増殖が著しく阻害された。 |
| Cas No. | 1219810-16-8 | SDF | |
| 同義語 | (1S,3R)-RSL3 | ||
| Chemical Name | (1S,3R)-methyl 2-(2-chloroacetyl)-1-(4-(methoxycarbonyl)phenyl)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylate | ||
| Canonical SMILES | COC(C1=CC=C([C@@]2([H])C3=C(C4=CC=CC=C4N3)C[C@](N2C(CCl)=O)([H])C(OC)=O)C=C1)=O | ||
| Formula | C23H21ClN2O5 | M.Wt | 440.88 |
| 溶解度 | ≥ 125.4 mg/mL in DMSO | Storage | Store at -20°C, sealed storage, away from moisture |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2682 mL | 11.341 mL | 22.6819 mL |
| 5 mM | 453.6 μL | 2.2682 mL | 4.5364 mL |
| 10 mM | 226.8 μL | 1.1341 mL | 2.2682 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Inhibition of APE1 promotes ferroptosis in HCC cells. A, B CCK-8 assay of cell viability in control and APE1-KD HepG2 and Huh7 cells after treatment with different concentrations of erastin and RSL3 for 24 h.
E3330, RSL-3, Z-VAD-FMK, Necrostatin 2, Chloroquine, and AR-A014418 were obtained from GlpBio (Montclair, CA,USA).
Cell Death & Differentiation (2024): 1-16. PMID: 38418695 IF: 12.4002 -
Related Biological Data
STAT6 attenuated LPS-induced ferroptosis with the improvement of SLC7A11 expression.(G-I) HBE cells were transfected with sh-Ctrl or sh-STAT6 and then treated with Erastin or RSL3.
Deferoxamine (DFO, GC13554),Erastin (GC16630), and RSL3 (GC12431) were purchased from Glpbio (Shanghai, China).
Cell Death & Disease 13.6 (2022): 1-14. PMID: 35668064 IF: 8.4685 -
Related Biological Data
Effects of different incubation patterns of 1,25(OH)2D3 on survival rate of ZFL after being treated with 3 uM RSL3 for 6 h (n = 6).
0-R (positive control group): First 1,25(OH)2D3-free for 72 h, then 3 uM RSL3 for 6 h;0-V-R (1,25(OH)2D3 preincubated for 6 h): First 1,25(OH)2D3-free for 66 h, then 200 pM 1,25(OH)2D3 for 6 h, and then 3 uM RSL3 for 6 h;V-R (1,25(OH)2D3 preincubated for 72 h): First 200 pM 1,25(OH)2D3 for 72 h, then 3 uM RSL3 for 6 h;
Int J Mol Sci 22.21 (2021): 11334. PMID: 34768761 IF: 5.924 -
Related Biological Data
Effects of 1,25(OH)2D3/VD3 on survival rate, morphology and mitochondrial membrane potential of zebrafish. (A) Effect of 1,25(OH)2D3 on the survival of larval zebrafish after RSL3 treatment (n = 60).
0+RSL3: egg water 72 h + 10 μM RSL3 24 h; 1,25(OH)2D3+RSL3: 50 nM 1,25(OH)2D3 72 h + 10 μM RSL3 24 h; Fer-1+RSL3:1 μM Fer-1 72 h + 10 μM RSL3 24 h.
Chemico-Biological Interactions (2023): 110828. PMID: 38081571 IF: 5.1
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)