Oligomycin Complex |
Katalog-Nr.GC16533
One-Click Copy Product Info
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Oligomycin Complex besteht aus einer Mischung von Oligomycin A, B und C.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1404-19-9
Sample solution is provided at 25 µL, 10mM.
Oligomycin Complex besteht aus einer Mischung von Oligomycin A, B und C. Oligomycin ist eine Klasse ähnlicher 26-gliedriger Makrolid-Antibiotika, die aus Streptomyces amylase chromogenes isoliert wurden. Oligomycin hat antimykotische, hemmende und insektizide Eigenschaften[1]. Oligomycin A hemmt primär die mitochondriale oxidative Phosphorylierung durch Blockierung des Protonenkanals(F0-Untereinheit) der ATP-Synthase und hemmt so die ATP-Synthese. Außerdem reduziert es den kompensatorischen Anstieg der oxidativen Phosphorylierung in Tumorzellen, was zu einer Senkung der zellulären Glykolyse führt[2-4]. Oligomycin B wirkt als nicht-selektiver Inhibitor der ATP-Synthasen und verlangsamt effektiv den ATP-Abbau bei Myokardischämie. Oligomycin C ist ein Inhibitor der mitochondrialen F1F0-ATP-Synthase, der einen empfindlicheren Zelltod induziert und das Zelltodmuster von Apoptose zu Nekrose verschiebt.
Oligomycin(0-50 µg/ml; 24 Stunden) induzierte in HepG2- und R-HepG2-Zellen eine Depolarisation der mitochondrialen Membran und die Freisetzung von Cytochrom c[5]. Oligomycin A(0-16 µM; 12-48 Stunden) fördert das Überleben und die Proliferation von HT29-Zellen[6]. In 549-Zellen konnte Oligomycin(100 ng/ml) die OXPHOS-Phosphorylierungsaktivität innerhalb einer Stunde vollständig hemmen und die Glykolyse in unterschiedlichem Ausmaß stimulieren[7]. Oligomycin(5 µg/ml) konnte den F0-Teil der H+-ATP-Synthase hemmen und die durch TNF induzierte Cytochrom-c-Freisetzung und Apoptose in HeLa-Zellen stark unterdrücken[8]. Oligomycin B(1 µM) induzierte Vasodilatationen, die in Sulfonylharnstoff-Rezeptor 2 Null(SUR2(nl)) um 39-61% geringer waren als in SUR2-Wildtyp(SUR2(wt))[9].
References:
[1]. SMITH RM, PETERSON WH, et,al.Oligomycin, a new antifungal antibiotic. Antibiot Chemother (Northfield). 1954 Sep;4(9):962-70. PMID: 24543225.
[2]. Vestergaard M, NØhr-Meldgaard K, et al. Inhibition of the ATP synthase eliminates the intrinsic resistance of Staphylococcus aureustowards polymyxins[J].mBio, 2017, 8(5): e01114-17.
[3]. Untereiner AA, Pavlidou A, et al. Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells[J].Biochem Pharmacol, 2018, 149: 174-185.
[4]. Ruas JS, Siqueira-Santos ES, et al. High glycolytic activity of tumor cells leads to underestimation of electron transport system capacity when mitochondrial ATP synthase is inhibited[J].Sci Rep, 2018, 8(1): 17383.
[5]. Li YC, Fung KP, et,al. Mitochondria-targeting drug oligomycin blocked P-glycoprotein activity and triggered apoptosis in doxorubicin-resistant HepG2 cells. Chemotherapy. 2004 Jun;50(2):55-62. doi: 10.1159/000077803. PMID: 15211078.
[6]. Li X, Tian R, et,al. Oligomycin A promotes radioresistance in HT29 colorectal cancer cells and its mechanisms. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Feb 28;46(2):113-120. English, Chinese. doi: 10.11817/j.issn.1672-7347.2021.200063. PMID: 33678646.
[7]. Hao W, Chang CP, et al. Oligomycin-induced bioenergetic adaptation in cancer cells with heterogeneous bioenergetic organization. J Biol Chem. 2010 Apr 23;285(17):12647-54. doi: 10.1074/jbc.M109.084194. Epub 2010 Jan 28. PMID: 20110356; PMCID: PMC2857128.
[8]. Shchepina LA, Pletjushkina OY, et al. Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. Oncogene. 2002 Nov 21;21(53):8149-57. doi: 10.1038/sj.onc.1206053. PMID: 12444550.
[9]. Adebiyi A, McNally EM, et al. Vasodilation induced by oxygen/glucose deprivation is attenuated in cerebral arteries of SUR2 null mice. Am J Physiol Heart Circ Physiol. 2011 Oct;301(4):H1360-8. doi: 10.1152/ajpheart.00406.2011. Epub 2011 Jul 22. PMID: 21784985; PMCID: PMC3197357.
| Zellexperiment 1[1]: | |
Zelllinien | R-HepG2- und HepG2-Zellen |
Vorbereitungsmethode | Die Zellen wurden mit Oligomycin in der angegebenen Konzentration oder mit Medium allein bei 37°C und 5% CO2 für 24 Stunden behandelt. |
Reaktionsbedingungen | 0-50 μg/ml; 24 Stunden |
Anwendungen | Oligomycin induzierte eine mitochondriale Depolarisation in R-HepG2- und HepG2-Zellen. |
| Zellexperiment 2[2]: | |
Zelllinien | HT29-Zelle |
Vorbereitungsmethode | Zellen wurden mit verschiedenen Konzentrationen von Oligomycin A für 12, 24, 36 und 48 Stunden kultiviert. |
Reaktionsbedingungen | 0-16 μM; 12-48 Stunden |
Anwendungen | Oligomycin fördert das Überleben und die Proliferation von HT29-Zellen. |
References: [1]. Li YC, Fung KP, et,al. Mitochondria-targeting drug oligomycin blocked P-glycoprotein activity and triggered apoptosis in doxorubicin-resistant HepG2 cells. Chemotherapy. 2004 Jun;50(2):55-62. doi: 10.1159/000077803. PMID: 15211078. [2]. Li X, Tian R, et,al. Oligomycin A promotes radioresistance in HT29 colorectal cancer cells and its mechanisms. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Feb 28;46(2):113-120. English, Chinese. doi: 10.11817/j.issn.1672-7347.2021.200063. PMID: 33678646. | |
| Cas No. | 1404-19-9 | SDF | |
| Canonical SMILES | O=C([C@H]([C@@H]([C@H](C([C@](C)([C@@H]([C@H](C/C=C/C=C/[C@]([H])(CC[C@@]1([C@@H]([C@]([H])(O2)[C@H]([C@@]3(O1)C(C[C@H]([C@]([H])(O3)C[C@H](C)O)C)=O)C)C)[H])CC)C)O)O)=O)C)O)C)[C@@H]([C@H]([C@@H](/C=C/C2=O)C)O)C.O=C([C@H]([C@@H]([C@H](C([C@](C)([C@@H]([C@H | ||
| Formula | C45H74O11 for Oligomycin A; C45H72O12 for Oligomycin B; C45H74O10 for Oligomycin C. | M.Wt | 791.06 for Oligomycin A; 805.1 for Oligomycin B; 775.1 for Oligomycin C. |
| Löslichkeit | ≤30mg/ml in ethanol;20mg/ml in DMSO;30mg/ml in dimethyl formamide | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2641 mL | 6.3206 mL | 12.6413 mL |
| 5 mM | 252.8 μL | 1.2641 mL | 2.5283 mL |
| 10 mM | 126.4 μL | 632.1 μL | 1.2641 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)