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(-)-Epigallocatechin gallate (EGCG) (Synonyms: EGCG)

カタログ番号GC14049

(-)-Epigallocatechin gallate (EGCG)は緑茶に含まれる主要なポリフェノール、細胞増殖を阻害でき、アポトーシスを誘発できる。

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(-)-Epigallocatechin gallate (EGCG) 化学構造

Cas No.: 989-51-5

サイズ 価格 在庫数 個数
10mM (in 1mL DMSO)
$39.00
在庫あり
100mg
$39.00
在庫あり
500mg
$144.00
在庫あり

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Sample solution is provided at 25 µL, 10mM.

Description of (-)-Epigallocatechin gallate (EGCG)

(-)-Epigallocatechin gallate (EGCG)は緑茶に含まれる主要なポリフェノール、細胞増殖を阻害でき、アポトーシスを誘発できる[1-2]。EGCGにはフリーラジカル消去特性がある[3]。EGCGはニューロンの酸化ストレスに対して抗炎症作用および抗アポトーシス作用を持っている[4]。なお、グルタミン酸デヒドロゲナーゼ1/2(GDH1/2、GLUD1/2)の活性も阻害する[5]。

(-)-Epigallocatechin gallate (EGCG)(200 µM;1h)はCoCl2が処理したBV2細胞における炎症誘発性サイトカインおよびメディエーターの発見レベルを下方修正する[6]。EGCGはmiR-143/MAPK7経路を通じて3T3-L1細胞の増殖を阻害する[7]。

(-)-Epigallocatechin gallate (EGCG)(5 -30 mg/kg;注射;14 日間)は腫瘍(HT-29結腸直腸がん)の成長を阻害する[8]。 EGCG治療(1日あたり50mg/kg)はを7日目から11日目まで家壁蝨(HDM)攻撃の1時間前に腹腔内注射によって、投与する) HIF-1α/VEGFA媒介性を阻害することにより鼠の喘息症状マクロファージのM2の傾動を軽減する[9]。

References:
[1]. Jin CF, Shen SR Sr, et,al. Different effects of five catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. J Agric Food Chem. 2001 Dec;49(12):6033-8. doi: 10.1021/jf010903r. PMID: 11743804.
[2]. De Amicis F, Perri A, et,al. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Oct;228(10):2054-62. doi: 10.1002/jcp.24372. PMID: 23553645.
[3]. Kondo K, Kurihara M, et,al. Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action. Free Radic Biol Med. 1999 Oct;27(7-8):855-63. doi: 10.1016/s0891-5849(99)00133-1. PMID: 10515590.
[4]. Schroeder EK, Kelsey NA, et,al. Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons. Antioxid Redox Signal. 2009 Mar;11(3):469-80. doi: 10.1089/ars.2008.2215. PMID: 18754708.
[5]. Peeters TH, Lenting K, et,al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4. doi: 10.1186/s40170-019-0198-7. PMID: 31139406; PMCID: PMC6526618.
[6]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.
[7]. Chen CP, Su TC, et,al. Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways. Exp Biol Med (Maywood). 2022 Sep;247(18):1670-1679. doi: 10.1177/15353702221108925. Epub 2022 Jul 27. PMID: 35894140; PMCID: PMC9597208.
[8]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123.
[9]. Yang N, Li X. Epigallocatechin gallate relieves asthmatic symptoms in mice by suppressing HIF-1α/VEGFA-mediated M2 skewing of macrophages. Biochem Pharmacol. 2022 Aug;202:115112. doi: 10.1016/j.bcp.2022.115112. Epub 2022 May 29. PMID: 35640712.

Protocol of (-)-Epigallocatechin gallate (EGCG)

Cell experiment []:

細胞株

BV2細胞

準備方法

細胞をEGCG (200 µM)で1時間処理し、CoCl2 (350 µM) に8時間暴露させる。

反応条件

200 µM; 1時間

アプリケーション

(-)-Epigallocatechin gallate (EGCG)はCoCl2が処理したBV2細胞における炎症誘発性サイトカインおよびメディエーターの発見レベルを下方修正する。

Animal experiment []:

動物モデル

BALB/cヌード鼠(HT-29結腸直腸がん)

準備方法

治療群では5 -20 mg/kgのEGCGは胃内投与し、対照群では100 uLの生理食塩水は胃内投与する、一日一回、14日間続く。

投与形態

5 -30 mg/kg; i.g; 14 日間

アプリケーション

(-)- Epigallocatechin Gallate(EGCG)腫瘍の成長を阻害する。

References:
[1]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-?B Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.
[2]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123.

Chemical Properties of (-)-Epigallocatechin gallate (EGCG)

Cas No. 989-51-5 SDF
同義語 EGCG
Chemical Name [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate
Canonical SMILES C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
Formula C22H18O11 M.Wt 458.37
溶解度 ≥ 22.9mg/mL in DMSO; ≥ 20mg/mL in Water Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of (-)-Epigallocatechin gallate (EGCG)

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1 mg 5 mg 10 mg
1 mM 2.1816 mL 10.9082 mL 21.8164 mL
5 mM 0.4363 mL 2.1816 mL 4.3633 mL
10 mM 0.2182 mL 1.0908 mL 2.1816 mL
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