MEGA-8 (Synonyms: N-octanoyl-N-Methylglucamine) |
Catalog No.GC44152
One-Click Copy Product Info
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MEGA-8은 octanoyl (C8 스트레이트 체인 아실)그룹과 N-methylglucamine의 축합에 의해 얻어지는 비이온성 글루코사이드 계면활성제이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85316-98-9
Sample solution is provided at 25 µL, 10mM.
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MEGA-8은 octanoyl (C8 스트레이트 체인 아실)그룹과 N-methylglucamine의 축합에 의해 얻어지는 비이온성 글루코사이드 계면활성제이다. MEGA-8은 5–40°C 범위에서 온도에 거의 영향을 받지 않는 임계 미셀 농도 (CMC)를 특징으로 하고 염화물 없는 상태에서는 CMC가 70 mM이고 고염 및 저염 상태에서는 5–64 mM이다[2][3]. MEGA-8은 일반적으로 생물 재료나 유기 화합물의 제조에 사용되고 단백질 추출과 막 단백질 용해와 같은 생명과학 연구에서 일반적으로 발견된다[4][5]. CHAPS와 결합할 때 MEGA-8은 CMC를 시너지적으로 감소시키고 표면 활성을 증강해서 온화한 조건하에서 막 단백질을 효율적으로 용해한다[6]. MEGA-8은 PrBP/δ를 부드럽게 배치하고 PDE6의 기초 활성을 억제하면서 호르몬 효소 복합체의 완정성과 균일성을 유지해서 망막 인산디에스테라제 6의 정제와 구조 연구에 이상적인 세제 환경을 제공한다[7].
References:
[1] Jeffrey GA, Maluszynska H. The structures of 1-deoxy-(N-methyloctanamido)-D-glucitol (MEGA-8) and 1-deoxy-(N-methylundecanamido)-D-glucitol (MEGA-11). Acta Crystallogr B. 1989;45 ( Pt 4):447-452.
[2] Walter A, Suchy SE, Vinson PK. Solubility properties of the alkylmethylglucamide surfactants. Biochim Biophys Acta. 1990;1029(1):67-74.
[3] Miyagishi S, Okada K, Asakawa T. Salt Effect on Critical Micelle Concentrations of Nonionic Surfactants, N-Acyl-N-methylglucamides (MEGA-n). J Colloid Interface Sci. 2001;238(1):91-95.
[4] Luescher IF, Unanue ER. Purification and photoaffinity labeling of the I-Ak histocompatibility molecule. J Immunol Methods. 1990;135(1-2):233-245.
[5] Banerjee P, Joo JB, Buse JT, Dawson G. Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem Phys Lipids. 1995;77(1):65-78.
[6] Ko JS, Oh SW, Kim KW, Nakashima N, Nagadome S, Sugihara G. Blending effects on adsorption and micellization of different membrane protein solubilizers: a thermodynamic study on three mixed systems of CHAPS with MEGA-8, -9 and -10 in pH 7.2 phosphate buffer solution. Colloids Surf B Biointerfaces. 2005;45(2):90-103.
[7] Baker BY, Palczewski K. Detergents stabilize the conformation of phosphodiesterase 6. Biochemistry. 2011;50(44):9520-9531.
| Cas No. | 85316-98-9 | SDF | |
| Synonyms | N-octanoyl-N-Methylglucamine | ||
| Chemical Name | 1-deoxy-1-[methyl(1-oxooctyl)amino]-D-glucitol | ||
| Canonical SMILES | OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CN(C)C(CCCCCCC)=O | ||
| Formula | C15H31NO6 | M.Wt | 321.4 |
| Solubility | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:2): 0.33 mg/ml,Ethanol: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1114 mL | 15.5569 mL | 31.1139 mL |
| 5 mM | 622.3 μL | 3.1114 mL | 6.2228 mL |
| 10 mM | 311.1 μL | 1.5557 mL | 3.1114 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)