MEGA-8 (Synonyms: N-octanoyl-N-Methylglucamine) |
Katalog-Nr.GC44152
One-Click Copy Product Info
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MEGA-8 is a nonionic glucoside surfactant obtained by condensation of octanoyl (C8 straight-chain acyl) group with N-methylglucamine.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85316-98-9
Sample solution is provided at 25 µL, 10mM.
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MEGA-8 is a nonionic glucoside surfactant obtained by condensation of octanoyl (C8 straight-chain acyl) group with N-methylglucamine[1]. MEGA-8 is characterized by its mildness and non-denaturing nature, with a critical micelle concentration (CMC) that is virtually unaffected by temperature in the range of 5–40 °C, and its CMC is 70 mM in salt-free conditions and 5–64 mM under high- and low-salt conditions[2][3]. MEGA-8 is usually used in the preparation of biomaterials or organic compounds and is commonly found in life science research, such as protein extraction and membrane protein solubilization[4][5]. When combined with CHAPS, MEGA-8 synergistically reduces the CMC and enhances surface activity, thereby efficiently solubilizing membrane proteins under mild conditions[6]. MEGA-8 gently displaces PrBP/δ and inhibits the basal activity of PDE6 while maintaining the integrity and homogeneity of the holoenzyme complex, thus providing an ideal detergent environment for the purification and structural studies of retinal phosphodiesterase 6[7].
References:
[1] Jeffrey GA, Maluszynska H. The structures of 1-deoxy-(N-methyloctanamido)-D-glucitol (MEGA-8) and 1-deoxy-(N-methylundecanamido)-D-glucitol (MEGA-11). Acta Crystallogr B. 1989;45 ( Pt 4):447-452.
[2] Walter A, Suchy SE, Vinson PK. Solubility properties of the alkylmethylglucamide surfactants. Biochim Biophys Acta. 1990;1029(1):67-74.
[3] Miyagishi S, Okada K, Asakawa T. Salt Effect on Critical Micelle Concentrations of Nonionic Surfactants, N-Acyl-N-methylglucamides (MEGA-n). J Colloid Interface Sci. 2001;238(1):91-95.
[4] Luescher IF, Unanue ER. Purification and photoaffinity labeling of the I-Ak histocompatibility molecule. J Immunol Methods. 1990;135(1-2):233-245.
[5] Banerjee P, Joo JB, Buse JT, Dawson G. Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem Phys Lipids. 1995;77(1):65-78.
[6] Ko JS, Oh SW, Kim KW, Nakashima N, Nagadome S, Sugihara G. Blending effects on adsorption and micellization of different membrane protein solubilizers: a thermodynamic study on three mixed systems of CHAPS with MEGA-8, -9 and -10 in pH 7.2 phosphate buffer solution. Colloids Surf B Biointerfaces. 2005;45(2):90-103.
[7] Baker BY, Palczewski K. Detergents stabilize the conformation of phosphodiesterase 6. Biochemistry. 2011;50(44):9520-9531.
| Cas No. | 85316-98-9 | SDF | |
| Überlieferungen | N-octanoyl-N-Methylglucamine | ||
| Chemical Name | 1-deoxy-1-[methyl(1-oxooctyl)amino]-D-glucitol | ||
| Canonical SMILES | OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CN(C)C(CCCCCCC)=O | ||
| Formula | C15H31NO6 | M.Wt | 321.4 |
| Löslichkeit | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:2): 0.33 mg/ml,Ethanol: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1114 mL | 15.5569 mL | 31.1139 mL |
| 5 mM | 622.3 μL | 3.1114 mL | 6.2228 mL |
| 10 mM | 311.1 μL | 1.5557 mL | 3.1114 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)