3,3′-Dipropylthiadicarbocyanine (iodide) (Synonyms: DiS-C3-(5), DiSC3(5); DiSC3(5)) |
Catalog No.GC52109
One-Click Copy Product Info
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3,3′-Dipropylthiadicarbocyanine (iodide) is a fluorescent probe often used as a tracer dye to assess the mitochondrial membrane potential.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 53213-94-8
Sample solution is provided at 25 µL, 10mM.
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DiSC3(5) is a fluorescent probe commonly used as a tracer dye to evaluate mitochondrial membrane potential. The excitation/emission wavelength of DiSC3(5) is up to 622/670 nm. DiSC3(5) can inhibit the respiratory system associated with mitochondrial NAD, and the IC50 value is 8 μM. DiSC3(5) in the presence of Na+/K+-ATPase inhibitor ouabain 2 can induce membrane hyperpolarization of Ehrlich ascites tumor cells[1][2][3].
References:
[1]. Yamamoto T, et al. Multiple effects of DiS-C3(5) on mitochondrial structure and function. Eur J Biochem. 2004;271(17):3573-3579.
[2]. Sims PJ, et al. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry. 1974 Jul 30;13(16):3315-30.
[3]. Smith TC, et al. The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells. Biochem Biophys Res Commun. 1980 Jul 31;95(2):722-7.
1. Preparation of DiSC3(5) Membrane Staining Solution:
1.1 Preparation of DMSO or EtOH Stock Solution: The stock solution should be prepared at a concentration of 1-5 mM in DMSO or EtOH.
Note 1): Unused portions of the stock solution should be stored at -20°C. Avoid repeated freeze-thaw cycles.
1.2 Preparation of Working Solution: Dilute the stock solution in a suitable buffer, such as serum-free culture medium, HBSS, or PBS, to prepare a working solution of 1 to 5 μM.
Note 2): The concentration of the working solution should be determined empirically for different cell types and/or experimental conditions. Testing a range of concentrations, at least tenfold, is recommended.
2. Staining Cells in Suspension:
2.1 Suspend cells at a density of 1 × 10⁶/mL in the dye working solution.
2.2 Incubate at 37°C for 2-20 minutes. Incubation time depends on the cell type. Start with a 20-minute incubation and then optimize as needed to obtain uniform labeling.
2.3 Centrifuge the labeled suspension tube at 1000 to 1500 rpm for 5 minutes.
2.4 Remove the supernatant and gently resuspend the cells in pre-warmed (37°C) growth medium.
2.5 Wash twice following steps 2.3 and 2.4.
3. Staining Adherent Cells:
3.1 Culture adherent cells on sterile glass coverslips.
3.2 Remove the coverslip from the growth medium and gently drain off excess medium. Place the coverslip in a humidified chamber.
3.3 Pipette 100 μL of the dye working solution onto the corner of the coverslip and gently swirl until all cells are covered.
3.4 Incubate the coverslip at 37°C for 2-20 minutes. Incubation time varies depending on the cell type. Start with a 20-minute incubation and then optimize as needed to obtain uniform labeling.
3.5 Drain off the dye working solution and wash the coverslip two to three times with growth medium. For each washing cycle, cover the cells with pre-warmed growth medium, incubate for 5-10 minutes, and then remove the medium.
4. Microscopic examination.
5. Flow cytometry analysis:
DiS-labeled cells can be analyzed using the standard FL3 flow cytometry detection channel.
| Cas No. | 53213-94-8 | SDF | |
| Synonyms | DiS-C3-(5), DiSC3(5); DiSC3(5) | ||
| Canonical SMILES | CCCN1C(SC2=C1C=CC=C2)=CC=CC=CC3=[N+](CCC)C(C=CC=C4)=C4S3.[I-] | ||
| Formula | C25H27IN2S2 | M.Wt | 546.53 |
| Solubility | DMSO : 5.83 mg/mL (10.67 mM; ultrasonic and warming and heat to 60°C) | Storage | 4°C, sealed storage, away from moisture and light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8297 mL | 9.1486 mL | 18.2973 mL |
| 5 mM | 365.9 μL | 1.8297 mL | 3.6595 mL |
| 10 mM | 183 μL | 914.9 μL | 1.8297 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)