5-Me-CTP,100mM Sodium Solution |
Catalog No.GB20005
One-Click Copy Product Info
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5-Me-CTP,100mM Sodium Solution is a modified nucleoside triphosphate that is commonly used to replace naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis, increasing mRNA stability and protein expression while reducing the interaction between innate immune receptors and immune cells.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 327174-86-7
Sample solution is provided at 25 µL, 10mM.
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5-Me-CTP,100mM Sodium Solution is a modified nucleoside triphosphate that is commonly used to replace naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis, increasing mRNA stability and protein expression while reducing the interaction between innate immune receptors and immune cells[1]. 5-Me-CTP is the only naturally occurring modified base in animal DNA and is found in mRNA, rRNA, and tRNA of various representative organisms[2]. Methylation of the fifth C atom of 5-Me-CTP is a reversible epigenetic modification that can regulate the binding of transcription factors or induce the binding of specific 5-mC binding proteins when present near gene regulatory regions, leading to the recruitment of co-repressor complexes to methylated target promoters[3]. 5-Me-CTP modification of RNA affects the fate of RNA molecules, including promoting mRNA stability, splicing, and nucleocytoplasmic transport; viral protein expression; DNA damage repair; cell tolerance, proliferation, and migration; and stem cell development, differentiation, and reprogramming[3]. The distribution of 5-Me-CTP varies among cell types, and 5-Me-CTP modifications at specific locations in mRNA exhibit different regulatory activities[4].
References:
[1] Steinle H, Ionescu T M, Schenk S, et al. Incorporation of synthetic mRNA in injectable chitosan-alginate hybrid hydrogels for local and sustained expression of exogenous proteins in cells[J]. International Journal of Molecular Sciences, 2018, 19(5): 1313.
[2] Riggs A D, Jones P A. 5-methylcytosine, gene regulation, and cancer[J]. Advances in cancer research, 1983, 40: 1-30.
[3] Breiling A, Lyko F. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond[J]. Epigenetics & chromatin, 2015, 8: 1-9.
[4] Song H, Zhang J, Liu B, et al. Biological roles of RNA m5C modification and its implications in Cancer immunotherapy[J]. Biomarker research, 2022, 10(1): 15.
| Cas No. | 327174-86-7 | SDF | |
| Formula | C10H15N3Na3O14P3 | M.Wt | 563.1 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7759 mL | 8.8794 mL | 17.7588 mL |
| 5 mM | 355.2 μL | 1.7759 mL | 3.5518 mL |
| 10 mM | 177.6 μL | 887.9 μL | 1.7759 mL |
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)