5-Me-CTP,100mM Sodium Solution

5-Methyl-CTP (5-Me-CTP) is a modified nucleoside triphosphate commonly used to replace naturally occurring CTP during in vitro mRNA synthesis, increasing mRNA stability and protein expression while reducing innate immunity Interaction of receptors with immune cells[1]. 5-Methylcytosine is the only naturally occurring modified base in animal DNA, and it is found in mRNA, rRNA and tRNA of various representative organisms[2]. 5-Methylcytosine fifth C-atom methylation is a reversible epigenetic modification that, when present near gene regulatory regions, can regulate the binding of transcription factors or induce the binding of specific 5 mC-binding proteins, resulting in co-repression The complex is recruited to methylated target promoters[3]. RNA 5-Me-CTP modification affects the fate of the modified RNA molecule, including promotion of mRNA stability, splicing and nucleoplasmic transport, DNA damage repair; mRNA stability; cell tolerance, proliferation and migration; stem cell development, Differentiation and reprogramming[3]. In addition, the distribution of 5-Me-CTP varies among cell types, and m5C modification at specific positions of mRNA exhibits different regulatory activities[4].

References:
[1]. Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62.
[2]. D. Riggs, Peter A. Jones. 5-Methylcytosine, Gene Regulation, and Cancer. Science, 1981 - science.org.
[3]. Achim Breiling.Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond. 2015 Jul 21;8:24. doi: 10.1186/s13072-015-0016-6. eCollection 2015.
[4]. Hang Song, et al. Biological roles of RNA m5C modification and its implications in Cancer immunotherapy. 2022 Apr 1;10(1):15. doi: 10.1186/s40364-022-00362-8.