77PD-Q (Synonyms: N,N'-bis(1,4-dimethylpentyl)-p-Phenylenediamine-quinone,N,N'-bis(1,4-dimethylpentyl)-para-Phenylenediamine-quinone) |
Catalog No.GC91737
One-Click Copy Product Info
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77PD-Q is a para-phenylenediamine quinone, which prevalently exists in the airborne particulates.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3078541-61-1
Sample solution is provided at 25 µL, 10mM.
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77PD-Q is a para-phenylenediamine quinone, which prevalently exists in the airborne particulates [1]. 77PD-Q induces endothelial-to-mesenchymal transition (EndMT) of cerebral microvascular endothelial cells by upregulating PAI-1, resulting in blood-brain barrier (BBB) dysfunction[2]. 77PD-Q has been widely used as a model compound to investigate the toxicity to aquatic organisms in sediment samples from urban rivers, coasts and deep-sea areas[3]. 77PD-Q has been widely used as an internal reference to optimize the conditions for ion chromatography and mass spectrometry analysis, thereby facilitating the identification and separation of related compounds respirable fine particulates[4].
In vitro, 77PD-Q (1μM) treatment for 24 hours significantly stimulated the release of inflammatory cytokines and lactate dehydrogenase (LDH) in hCMEC-D3 cells and promoted cell apoptosis[2].
References:
[1] Wang W, Cao G, Zhang J, et al. Fragmentation pattern-based screening strategy combining diagnostic ion and neutral loss uncovered novel para-phenylenediamine quinone contaminants in the environment[J]. Environmental Science & Technology, 2024, 58(13): 5921-5931.
[2] Han M, Xia K, Xue Y, et al. Emergence of more potent PPD-Qs beyond 6PPD-Q in human blood and cerebrospinal fluid[J]. Environmental Science & Technology Letters, 2025, 12(5): 496-502.
[3] Zeng L, Li Y, Sun Y, et al. Widespread occurrence and transport of p-phenylenediamines and their quinones in sediments across urban rivers, estuaries, coasts, and deep-sea regions[J]. Environmental science & technology, 2023, 57(6): 2393-2403.
[4] Wang W, Cao G, Zhang J, et al. Beyond substituted p-Phenylenediamine antioxidants: Prevalence of their quinone derivatives in PM2. 5[J]. Environmental science & technology, 2022, 56(15): 10629-10637.
| Cell experiment [1]: | |
Cell lines | hCMEC-D3 cells |
Preparation Method | hCMEC-D3 cells were grown in endothelial cell medium with 5% (v/v) fetal bovine serum (FBS), 1% endothelial cell growth supplement, and 1% penicillin/streptomycin solution in the presence of 5% CO2 at 37°C. Cells (5×103 cells/ml) were seeded in 96-well plates and allowed to adhere in a 5% CO2 incubator at 37°C overnight. Cells were treated with different concentrations of 77PD-Q (0, 0.01, 0.10, 1.00μM) for 24h. The cell viability was evaluated. |
Reaction Conditions | 0, 0.01, 0.10, 1.00μM; 24h |
Applications | 77PD-Q treatment significantly reduced cell viability of hCMEC-D3 cells in a dose-dependent manner. |
References: | |
| Cas No. | 3078541-61-1 | SDF | |
| Synonyms | N,N'-bis(1,4-dimethylpentyl)-p-Phenylenediamine-quinone,N,N'-bis(1,4-dimethylpentyl)-para-Phenylenediamine-quinone | ||
| Formula | C20H34N2O2 | M.Wt | 334.5 |
| Solubility | Acetonitrile: Slightly soluble: 0.1-1 mg/ml,Chloroform: Sparingly soluble: 1-10 mg/ml,Water: Slightly soluble: 0.1-1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9895 mL | 14.9477 mL | 29.8954 mL |
| 5 mM | 597.9 μL | 2.9895 mL | 5.9791 mL |
| 10 mM | 299 μL | 1.4948 mL | 2.9895 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)