Cy3-UTP, 10mM Sodium Solution (Synonyms: Aminoallyl-UTP - Cy3;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP) |
| Catalog No.GB20116 |
Cy3-UTP (Cyanine 3-UTP) can replace UTP as the substrate of T7RNA polymerase to produce labeled probes through in vitro transcription.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Cy3-UTP (Cyanine 3-UTP) can replace UTP as the substrate of T7RNA polymerase to produce labeled probes through in vitro transcription. Λexc=550nm/Λem=570nm[1]. Cy3 dye gives the UTP molecule fluorescent properties, and Cy3-UTP can be incorporated into RNA molecules through the transcription reaction catalyzed by RNA polymerase. Enables researchers to track RNA distribution, localization and dynamics[2]. Cy3-UTP labeled mRNA is easily visible under UV light and does not require further staining after gel electrophoresis [3]. Cy3-UTP can be used for single-molecule fluorescence resonance energy transfer (FRET) measurement to monitor the co-transcriptional folding of RNA molecules in real time [4].This product is provided as a 10mM aqueous solution.
References:
[1]Bao W , Li A , Zhang Y ,et al. Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata[J].Plant Biotechnology Journal, 2021.
[2]Erika Schaudy,Jory Lietard, Mark M Somoza. Enzymatic Synthesis of High-Density RNA Microarrays[J]. Nucleic Acid Chemistry section. 2023 Feb;3(2):e667. doi: 10.1002/cpz1.667.
[3]Kim H, Park Y, Lee J B. Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression[J]. Scientific Reports, 2015, 5:12737.
[4]Uhm H , Kang W , Ha K S ,et al. Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch[J].Proceedings of the National Academy of Sciences, 2017, 115(2):201712983.
| Cas No. | SDF | ||
| Synonyms | Aminoallyl-UTP - Cy3;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP | ||
| Chemical Name | 2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylid | ||
| Canonical SMILES | O=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([C@H]6[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O | ||
| Formula | C43H56N5O22P3S2(free acid) | M.Wt | 1151.98 (free acid) |
| Solubility | Soluble in H2O | Storage | Store at -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 868.1 μL | 4.3404 mL | 8.6807 mL |
| 5 mM | 173.6 μL | 868.1 μL | 1.7361 mL |
| 10 mM | 86.8 μL | 434 μL | 868.1 μL |
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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