Cy3-UTP, 10mM Sodium Solution (Synonyms: Aminoallyl-UTP - Cy3;5-(3-Aminoallyl)-uridine-5'-triphosphate; labeled with Cy3;Triethylammonium salt;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP) |
Catalog No.GB20116 |
Cy3-UTP (Cyanine3-UTP) can replace UTP as the substrate of T7 RNA polymerase to produce labeled probes through in vitro transcription. Λexc=550nm/Λem=570nm..
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Cy3-UTP (Cyanine 3-UTP) can replace UTP as the substrate of T7RNA polymerase to produce labeled probes through in vitro transcription. Λexc=550nm/Λem=570nm[1]. Cy3 dye gives the UTP molecule fluorescent properties, and Cy3-UTP can be incorporated into RNA molecules through the transcription reaction catalyzed by RNA polymerase. Enables researchers to track RNA distribution, localization and dynamics[2]. Cy3-UTP labeled mRNA is easily visible under UV light and does not require further staining after gel electrophoresis [3]. Cy3-UTP can be used for single-molecule fluorescence resonance energy transfer (FRET) measurement to monitor the co-transcriptional folding of RNA molecules in real time [4].This product is provided as a 10mM aqueous solution.
References:
[1]Bao W , Li A , Zhang Y ,et al. Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata[J].Plant Biotechnology Journal, 2021.
[2]Erika Schaudy,Jory Lietard, Mark M Somoza. Enzymatic Synthesis of High-Density RNA Microarrays[J]. Nucleic Acid Chemistry section. 2023 Feb;3(2):e667. doi: 10.1002/cpz1.667.
[3]Kim H, Park Y, Lee J B. Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression[J]. Scientific Reports, 2015, 5:12737.
[4]Uhm H , Kang W , Ha K S ,et al. Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch[J].Proceedings of the National Academy of Sciences, 2017, 115(2):201712983.
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