DMANI |
Catalog No.GC28212
One-Click Copy Product Info
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DMANI is an intramolecular charge transfer (ICT)-based mitochondria-targeted ratiometric fluorescent probe.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2978635-30-0
Sample solution is provided at 25 µL, 10mM.
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DMANI is an intramolecular charge transfer (ICT)-based mitochondria-targeted ratiometric fluorescent probe. DMANI can monitor mitochondrial peroxynitrite (ONOO−) in living cells[1].
In PBS buffer (10 mM, pH = 7.4), the DMANI (5 μM) displays absorption bands at around 525 and 346 nm, but the addition of 30 μM ONOO− significantly decreased the absorption, together with the appearance of a new peak at about 360 nm. In the emission spectra, a remarkably decreased fluorescence was observed at 692 nm and a new emission band at 444 nm appeared and increased rapidly in the case of DMANI with ONOO−[1].
References:
[1]. Yuting Du, et al. ICT-based fluorescent ratiometric probe for monitoring mitochondrial peroxynitrite in living cells. New J. Chem., 2021,45, 12915-12921.
| Cas No. | 2978635-30-0 | SDF | |
| Chemical Name | 3H-Indolium, 2-[2-[6-(dimethylamino)-2-naphthalenyl]ethenyl]-1,3,3-trimethyl-, iodide (1:1) | ||
| Formula | C25H27IN2 | M.Wt | 482.40 |
| Solubility | DMSO : 100 mg/mL (207.30 mM; Need ultrasonic) | Storage | 4°C, sealed storage, away from moisture and light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.073 mL | 10.3648 mL | 20.7297 mL |
| 5 mM | 414.6 μL | 2.073 mL | 4.1459 mL |
| 10 mM | 207.3 μL | 1.0365 mL | 2.073 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)