EGF receptor substrate eps15 acetyl - [Mus musculus]/[Homo sapiens] (Synonyms: Ac-Gln-Glu-Gln-Glu-Asp-Leu-Glu-Leu-Ala-Ile-Ala-Leu-Ser-Lys-Ser-Glu-Ile-Ser-Glu-Ala-OH ) |
| Catalog No.GP10030 |
EGF receptor substrate eps15 (Eps15) has been identified as a 142-kDa substrate of the EGF receptor1.
Products are for research use only. Not for human use. We do not sell to patients.
![EGF receptor substrate eps15 acetyl - [Mus musculus]/[Homo sapiens] Chemical Structure](/media/struct/GP1/GP10030.png "EGF receptor substrate eps15 acetyl - [Mus musculus]/[Homo sapiens] Chemical Structure")
Sample solution is provided at 25 µL, 10mM.
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Sequence: AC-QEQEDLELAIALSKSEISEA-OH
Formula of EGF receptor substrate eps15 acetyl - [Mus musculus]/[Homo sapiens]:
C96H159N23O39
EGF receptor substrate eps15 (Eps15) has been identified as a 142-kDa substrate of the EGF receptor1. In quiescent cells Eps15 is associated to the EGF receptor, and upon EGF stimulation this association increases dramatically2. In addition, Eps15 has been shown to bind to both adaptor protein-2 and clathrin2, 3. Subcellular fractionation and immunolocalization studies have shown that Eps15 is present in clathrin-coated pits and vesicles but not in early endosomes2, 4. Eps15 shares homology with the yeast proteins End3p and Pan1p. Both proteins contain multiple Eps15 homology domains, a motif proposed to mediate proteinprotein interaction, and have been implicated in the endocytosis of the a-factor and lipids, respectively5, 6.
Eps15 can be directly phosphorylated in vitro by EGFR and is tyrosine phosphorylated at high stoichiometry in vivo following activation of the EGFR. Perturbation of Eps15 function affects cell proliferation. Overexpression of Eps15 can transform NIH 3T3 cells, albeit with low efficiency7.
Figure1.Structure of EGF receptor substrate eps15
Ref:
1. Fazioli, F., Minichiello, L., Matoskova, B., Wong, W. T., and Di Fiore, P. P. (1993) Mol. Cell. Biol. 13, 5814–5828
2. van Delft, S., Schumacher, C., Hage, W., Verkleij, A. J., and van Bergen en Henegouwen, P. M. P. (1997) J. Cell Biol. 136, 811–823
3. Benmerah, A., Gagnon, J., Be`gue, B., Me´garbane´, B., Dautry-Varsat, A., and Cerf-Bensussan, N. (1996) J. Cell Biol. 131, 1831–1838
4. Tebar, F., Sorkina, T., Sorkin, A., Ericsson, M., and Kirchhausen, T. (1996) J. Biol. Chem. 271, 28727–28730
5. Benedetti, H., Raths, S., Crausaz, F., and Riezman, H. (1994) Mol. Biol. Cell 5,1023–1037
6. Wendland, B., McCaffery, J. M., Xiao, Q., and Emr, S. D. (1996) J. Cell Biol. 135, 1485–1500
7. Fazioli, F., Minichiello, L., Matoskova, B., Wong, W. T. & Di Fiore, P. P. (1993) Mol. Cell. Biol. 13, 5814-5828.
| Cas No. | SDF | ||
| Synonyms | Ac-Gln-Glu-Gln-Glu-Asp-Leu-Glu-Leu-Ala-Ile-Ala-Leu-Ser-Lys-Ser-Glu-Ile-Ser-Glu-Ala-OH | ||
| Formula | C96H159N23O39 | M.Wt | 2259.42 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.4426 mL | 2.213 mL | 4.4259 mL |
| 5 mM | 0.0885 mL | 0.4426 mL | 0.8852 mL |
| 10 mM | 0.0443 mL | 0.2213 mL | 0.4426 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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