ER-Tracker Green |
Catalog No.GC64788
One-Click Copy Product Info
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ER-Tracker Green is a fluorescent dye that specific for endoplasmic reticulum.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 730931-46-1
Sample solution is provided at 25 µL, 10mM.
ER-Tracker Green is a fluorescent dye that specific for endoplasmic reticulum[1].
ER tracker Green (1 mM, incubated in OptiMEM for 30 min at 37 °C) is used for endoplasmic reticulum staining[1].
References:
[1]. J Jacob Strouse, et al. Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters. Anal Biochem. 2013 Jun 1;437(1):77-87.
This plan only provides a guide, please modify it to meet your specific needs.
1. Preparation of staining solution
(1) Prepare DMSO storage solution: Dissolve ER-Tracker Green in DMSO and prepare a storage solution with a concentration of 1mM.
Note: Unused storage solution should be aliquoted and stored in the dark at -20°C or -80°C to avoid repeated freezing and thawing.
(2) Prepare working solution: Dilute the storage solution with a suitable buffer (such as serum-free medium or PBS) and prepare a working solution with a concentration of 100 nM-1 μM.
Note: Please adjust the concentration of the working fluid according to the actual situation and prepare it now.
2. Cell suspension staining
(1) Suspension cells: Centrifuge at 1000g for 3-5 minutes at 4°C, discard the supernatant, and wash twice with PBS for 5 minutes each time.
(2) Adherent cells: Wash twice with PBS, add trypsin to digest the cells, and centrifuge at 1000g for 3-5 minutes after digestion is completed.
(3) Add 1mL of DiOC6 (3) iodide working solution and resuspend about 106 cells. Incubate at room temperature in dark for 5-30 minutes. The optimal incubation time for different cells is different, please explore by yourself according to the specific experimental needs.
(4) After the incubation, centrifuge at 1000g for 5 minutes, remove the supernatant, add PBS and wash 2-3 times, 5 minutes each time.
(5) Resuspend the cells in pre-warmed serum-free cell culture medium or PBS. Observe by fluorescence microscopy or flow cytometry.
3. Cell adhesion staining
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, suck out the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100μL of dye working solution from one corner of the coverslip and shake gently to evenly cover all cells with the dye.
(4) Incubate at room temperature in the dark for 5-30 minutes. The optimal incubation time for different cells is different, please explore by yourself according to the specific experimental needs.
(5) After the incubation, discard the dye working solution and use pre-warmed culture solution to wash the coverslip 2 to 3 times.
4. Microscope detection: The maximum excitation/emission light of ER-Tracker Green is 489/520 nm respectively.
Precautions:
① If you want to fix the stained cells, it is recommended to use 4% formaldehyde for 2 minutes at 37℃;
② If the stained cells need to be permeabilized, it is recommended to use ER–Tracker Blue–White DPX (GB30171);
③ Fluorescent dyes all have quenching problems. Please try to avoid light to slow down fluorescence quenching;
④ For your safety and health, please wear a lab coat and disposable gloves.
| Cas No. | 730931-46-1 | SDF | |
| Formula | C37H42BClF2N6O6S | M.Wt | 783.09 |
| Solubility | DMSO : 50 mg/mL (63.85 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.277 mL | 6.385 mL | 12.7699 mL |
| 5 mM | 255.4 μL | 1.277 mL | 2.554 mL |
| 10 mM | 127.7 μL | 638.5 μL | 1.277 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)