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FITC-Phalloidin

Catalog No.GC19997

Phalloidin is a cyclic heptapeptide toxin derived from Amanita phalloides, which selectively binds to filamentous actin F-actin with high affinity (Kd=20nM).

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FITC-Phalloidin Chemical Structure

Size Price Stock Qty
300T
$197.00
In stock
1mg
$1,282.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

FITC-bicyclic(Ala-DThr-Cys-cis-4-hydroxy-Pro-Ala-2mercapto-Trp-4-hydroxy-5-amino-Leu)(S-3 to 6).

Phalloidin is a cyclic heptapeptide toxin derived from Amanita phalloides, which selectively binds to filamentous actin F-actin with high affinity (Kd=20nM). , without binding to monomeric actin G-actin, is commonly used to label F-actin in tissue sections, cell cultures or cell-free systems for qualitative and quantitative analysis of F-actin, in addition, ghost pen Cyclic peptide derivatives also bind to large and small fibers with similar affinity, whether it is animal or plant derived muscle cells or non-muscle cells, according to the ratio of each actin subunit to about one phalloidin molecule, and it is not specific. The heterozygous binding is almost negligible, and the stained and non-stained areas are clearly distinguishable. Therefore, phalloidin derivatives are particularly suitable for replacing actin (Actin) antibodies for related studies. In addition, the phalloidin derivatives are very small, with a diameter of about 12-15A and a molecular weight of <2000 Daltons. Many physiological properties of unlabeled actin (Actin) are maintained, for example, with actin-binding proteins such as myosin. , tropomyosin, DNase1, etc. can still react; phalloidin-labeled filaments can still penetrate the solid-phase myosin matrix; and glycerol-extracted muscle fibers can still contract after labeling. The binding of phalloidin prevents the dissociation of filamentous actin (microfilaments) and stabilizes the microfilament structure, thereby disrupting the dynamic equilibrium of microfilament polymerization-depolymerization. This property reduces the critical concentration (CC) for actin polymerization to <1 pg/mL, and thus acts as a polymerization promoter, and phalloidin also inhibits the ATP hydrolysis activity of F-actin. This product is FITC-labeled phalloidin, which has strong staining specificity and high contrast, and has better staining effect than Actin antibody. It is suitable for qualitative and quantitative detection of F-actin. In addition, the F-actin combined with this product can still maintain many biological properties of actin itself. And the combination of this product has no species difference, and it has a wide range of applicability. Our company provides FITC-labeled phalloidin in the form of freeze-dried powder and storage solution, which users can choose according to their own needs. The recommended concentration is 80~200nM.

Chemical Properties

Cas No. SDF
Formula C56H60N10O15S56 M.Wt 1177.3 g/mol
Solubility Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

Review for FITC-Phalloidin

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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