Phalloidin is a cyclic heptapeptide toxin derived from Amanita phalloides, which selectively binds to filamentous actin F-actin with high affinity (Kd=20nM).
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Sample solution is provided at 25 µL, 10mM.
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FITC-bicyclic(Ala-DThr-Cys-cis-4-hydroxy-Pro-Ala-2mercapto-Trp-4-hydroxy-5-amino-Leu)(S-3 to 6).
Phalloidin is a cyclic heptapeptide toxin derived from Amanita phalloides, which selectively binds to filamentous actin F-actin with high affinity (Kd=20nM). , without binding to monomeric actin G-actin, is commonly used to label F-actin in tissue sections, cell cultures or cell-free systems for qualitative and quantitative analysis of F-actin, in addition, ghost pen Cyclic peptide derivatives also bind to large and small fibers with similar affinity, whether it is animal or plant derived muscle cells or non-muscle cells, according to the ratio of each actin subunit to about one phalloidin molecule, and it is not specific. The heterozygous binding is almost negligible, and the stained and non-stained areas are clearly distinguishable. Therefore, phalloidin derivatives are particularly suitable for replacing actin (Actin) antibodies for related studies. In addition, the phalloidin derivatives are very small, with a diameter of about 12-15A and a molecular weight of <2000 Daltons. Many physiological properties of unlabeled actin (Actin) are maintained, for example, with actin-binding proteins such as myosin. , tropomyosin, DNase1, etc. can still react; phalloidin-labeled filaments can still penetrate the solid-phase myosin matrix; and glycerol-extracted muscle fibers can still contract after labeling. The binding of phalloidin prevents the dissociation of filamentous actin (microfilaments) and stabilizes the microfilament structure, thereby disrupting the dynamic equilibrium of microfilament polymerization-depolymerization. This property reduces the critical concentration (CC) for actin polymerization to <1 pg/mL, and thus acts as a polymerization promoter, and phalloidin also inhibits the ATP hydrolysis activity of F-actin. This product is FITC-labeled phalloidin, which has strong staining specificity and high contrast, and has better staining effect than Actin antibody. It is suitable for qualitative and quantitative detection of F-actin. In addition, the F-actin combined with this product can still maintain many biological properties of actin itself. And the combination of this product has no species difference, and it has a wide range of applicability. Our company provides FITC-labeled phalloidin in the form of freeze-dried powder and storage solution, which users can choose according to their own needs. The recommended concentration is 80~200nM.
|Store at -20°C, protect from light
|Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
|Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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