1,2-Dimyristoyl-rac-glycero-3-phosphocholine |
Catalog No.GA20356
One-Click Copy Product Info
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1,2-Dimyristoyl-rac-glycero-3-phosphocholine, a zwitterionic phospholipid, was chosen as a simple but suitable membrane model of the human cell membrane for mimicking the neutral charge of the surface membrane of eukaryotic plasma membranes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 18656-38-7
Sample solution is provided at 25 µL, 10mM.
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1,2-Dimyristoyl-rac-glycero-3-phosphocholine, a zwitterionic phospholipid, was chosen as a simple but suitable membrane model of the human cell membrane for mimicking the neutral charge of the surface membrane of eukaryotic plasma membranes[1]. The effect of antibiotics such as Rifabutin on human cell membrane models was assessed using multilamellar vesicles (MLVs) composed of 1,2-Dimyristoyl-rac-glycero-3-phosphocholine[1-2]. 1,2-Dimyristoyl-rac-glycero-3-phosphocholine can serve as a model lipid membrane to study the effects of DMSO and drugs on eukaryotic cell membranes[3-5]. 1,2-Dimyristoyl-rac-glycero-3-phosphocholine can also be used to prepare polydiacetylene (PDA)[6]. 1,2-Dimyristoyl-rac-glycero-3-phosphocholine experiences an increase in headgroup area, enhanced membrane disorder, and increased fluidity due to DMSO, but the bilayer does not disintegrate[4].
References:
[1] Pinheiro M, Nunes C, Caio JM, et al. The influence of rifabutin on human and bacterial membrane models: implications for its mechanism of action. J Phys Chem B. 2013;117(20):6187-6193.
[2] Pinheiro M, Nunes C, Caio JM, Moiteiro C, Brezesinski G, Reis S. Interactions of N'-acetyl-rifabutin and N'-butanoyl-rifabutin with lipid bilayers: a synchrotron X-ray study. Int J Pharm. 2013;453(2):560-568.
[3] Kundu S, Malik S, Ghosh M, et al. A Comparative Study on DMSO-Induced Modulation of the Structural and Dynamical Properties of Model Bilayer Membranes. Langmuir. 2021;37(6):2065-2078.
[4] Sahu S, Garg A, Saini R, Debnath A. Interface Water Assists in Dimethyl Sulfoxide Crossing and Poration in Model Bilayer. Langmuir. 2024;40(11):5764-5775.
[5] Pinheiro M, Amenitsch H, Reis S. Antituberculosis Drug Interactions with Membranes: A Biophysical Approach Applied to Bedaquiline. Membranes (Basel). 2019;9(11):141.
[6] Jung YK, Park HG. Colorimetric detection of clinical DNA samples using an intercalator-conjugated polydiacetylene sensor. Biosens Bioelectron. 2015;72:127-132.
This plan only provides a guide, please modify it to meet your specific needs.
Preparation of 1,2-Dimyristoyl-rac-glycero-3-phosphocholine vesicles[1]：
1. Initially, the required amount of 1,2-Dimyristoyl-rac-glycero-3-phosphocholine lipid (∼2mg) was taken in the round-bottom flask and solubilized in a 1:1 chloroform/methanol mixture.
2. Subsequently, the mixture was slowly evaporated using a rotary evaporator to produce the thin film of dry lipid at the bottom of the flask.
3. The thin film was then hydrated using the required amount of water or DMSO/water mixtures prepared previously, and the volume was adjusted to achieve a final lipid concentration of ∼3mM.
4. For the spectroscopic studies, sonicate the vesicle solutions by means of an ultrasonic probe sonicator using a pulse of ∼20 ± 3kHz, followed by passing the vesicle solution through a 450nm pore size cellulose filter to obtain the uniform distribution of vesicles ∼100 ± 20nm in size.
5. Then the solutions were added to the methanol-evaporated Coumarin 153 (C153) or 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM) solution as required.
DMSO treatment of 1,2-Dimyristoyl-rac-glycero-3-phosphocholine[2]:
1. DMSO is used as a cosolvent, and its concentration is gradually increased from 0% to 25%.
Analysis of water dynamics in 1,2-Dimyristoyl-rac-glycero-3-phosphocholine after DMSO treatment[2]:
1. Lipids are modeled using the Slipid force field, water with the TIP4P/2005 model, and DMSO parameters are derived from General Amber Force Field.
2. PACKMOL is used to arrange lipids, water, and DMSO evenly within the bilayer.
3. Energy minimization is performed using the steepest-descent algorithm.
4. Simulations are conducted in the NPT ensemble with a temperature of 350K and a pressure of 1bar.
5. The bilayer is equilibrated for 800–1000ns with all-bond constraints, followed by 1μs of NPT simulation without bond constraints.
6. Bilayer properties such as area per lipid, density profile, and thickness are analyzed from the NPT production runs.
References:
[1] Kundu S, Malik S, Ghosh M, et al. A Comparative Study on DMSO-Induced Modulation of the Structural and Dynamical Properties of Model Bilayer Membranes. Langmuir. 2021;37(6):2065-2078.
[2] Sahu S, Garg A, Saini R, Debnath A. Interface Water Assists in Dimethyl Sulfoxide Crossing and Poration in Model Bilayer. Langmuir. 2024;40(11):5764-5775.
| Cas No. | 18656-38-7 | SDF | |
| Formula | C36H72NO8P | M.Wt | 677.94 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.4751 mL | 7.3753 mL | 14.7506 mL |
| 5 mM | 295 μL | 1.4751 mL | 2.9501 mL |
| 10 mM | 147.5 μL | 737.5 μL | 1.4751 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)