Cyclo(Arg-Gly-Asp-D-Phe-Cys) |
Catalog No.GA21303
One-Click Copy Product Info
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Cyclo(Arg-Gly-Asp-D-Phe-Cys), commonly abbreviated as cRGDfC, is a cyclic RGD peptide that shows high affinity and selectivity for the integrin αvβ3 .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 862772-11-0
Sample solution is provided at 25 µL, 10mM.
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Cyclo(Arg-Gly-Asp-D-Phe-Cys), commonly abbreviated as cRGDfC, is a cyclic RGD peptide that shows high affinity and selectivity for the integrin αvβ3 [1]. Treatment of murine embryonic stem cells (mESCs) with Cyclo(Arg-Gly-Asp-D-Phe-Cys) peptide can induce some cells to express early ectoderm lineage markers [2]. Cyclo(Arg-Gly-Asp-D-Phe-Cys) is mainly used to modify liposomes and other delivery systems to achieve targeted drug delivery to tumor tissues [3-4].
In murine embryonic stem cells, Cyclo(Arg-Gly-Asp-D-Phe-Cys) (0.5mM; 24 h) down-regulated the transcription factors Oct 4, Sox 2, and Nanog [2].
References:
[1]. Nallamothu R, Wood GC, Pattillo CB, et al. A tumor vasculature targeted liposome delivery system for combretastatin A4: design, characterization, and in vitro evaluation. Aaps Pharmscitech. 2006 Jun; 7: E7-16.
[2]. Hazenbiller O, Duncan NA, Krawetz RJ. Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide. BMC Cell Biology. 2017 Dec; 18: 1-5.
[3]. Chen Z, Deng J, Zhao Y, et al. Cyclic RGD peptide-modified liposomal drug delivery system: enhanced cellular uptake in vitro and improved pharmacokinetics in rats. International journal of nanomedicine. 2012 Jul 18: 3803-3811.
[4]. Xia Y, Chen Y, Hua L, et al. Functionalized selenium nanoparticles for targeted delivery of doxorubicin to improve non-small-cell lung cancer therapy. International Journal of Nanomedicine. 2018 Oct 30: 6929-6939.
| Cell experiment [1]: | |
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Cell lines |
Murine embryonic stem cells (mESCs) |
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Preparation Method |
Murine embryonic stem cells (mESCs) were embedded in a type I collagen matrix. Briefly, mESCs at passage 8 to 10 were trypsinized and re-suspended in β-glycerol phosphate medium (βGP). βGP medium consisted of high glucose 5 × DMEM powder medium supplemented with 10mM βGP and with FBS, NEAA, penicillin-stereptomycin solution, and βME equal to the standard cell culture procedure. To prepare one collagen construct, 1,000,000 cells were re-suspended in 200μL βGP medium and mixed with 800μL of bovine collagen type I solution (3mg/mL) and of 20μL sodium hydroxide. For the integrin studies, Cyclo(Arg-Gly-Asp-D-Phe-Cys) (cRGDfC) peptide was dissolved in 3% citric acid and this was supplemented to mESC-Col I solution to achieve a final concentration of 0.5mM cRGDfC. Finally, mESC-Col I solution, in the presence and absence of cRGDfC peptide, was pipetted into a 12 well plate and allowed to polymerize in a humidified incubator with 5% CO2 at 37℃. |
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Reaction Conditions |
0.5mM; 24h |
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Applications |
Cyclo(Arg-Gly-Asp-D-Phe-Cys) down-regulated the transcription factors Oct 4, Sox 2, and Nanog in mESCs. |
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References: |
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| Cas No. | 862772-11-0 | SDF | |
| Formula | C24H34N8O7S | M.Wt | 578.65 |
| Solubility | DMSO: 50 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7282 mL | 8.6408 mL | 17.2816 mL |
| 5 mM | 345.6 μL | 1.7282 mL | 3.4563 mL |
| 10 mM | 172.8 μL | 864.1 μL | 1.7282 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)