DABCYL-γ-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS |
| Catalog No.GA21362 |
The fluorogenic HIV-1 protease substrate DABCYL-Gaba-SQNYPIVQ-EDANS consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH?-termini. The γ-Abu spacer was inserted to avoid potential steric hindrance of substrate binding by the bulky acceptor. This FRET substrate is cleaved by the HIV-1 protease at the Tyr-Pro bond which results in a time-dependent increase in fluorescence intensity; excitation at 340 nm, emission at 490 nm.
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Cas No.: 127134-13-8
Sample solution is provided at 25 µL, 10mM.
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The fluorogenic HIV-1 protease substrate DABCYL-Gaba-SQNYPIVQ-EDANS consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH-termini. The γ-Abu spacer was inserted to avoid potenti
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