Glutaryl-Gly-Arg-AMC |
Catalog No.GA21910
One-Click Copy Product Info
|
Glutaryl-Gly-Arg-AMC is a fluorescent substrate and a peptide substrate of urokinase plasminogen activator (uPA). The maximum excitation/emission wavelength of the fluorophore AMC is 380nm/460nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 65147-16-2
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Glutaryl-Gly-Arg-AMC is a fluorescent substrate and a peptide substrate of urokinase plasminogen activator (uPA). The maximum excitation/emission wavelength of the fluorophore AMC is 380nm/460nm[1, 2]. Glutaryl-Gly-Arg-AMC can be used to detect protease activity, especially trypsin and trypsin-like enzymes[3, 4].
References:
[1] Wu G, Mazzitelli B A, Quek A J, et al. Tranexamic acid is an active site inhibitor of urokinase plasminogen activator[J]. Blood advances, 2019, 3(5): 729-733.
[2] Dewey S. Fluctuating Effects of Diabetes Mellitus Type 1 and 2 on the Ubiquitin Proteasome System in Two Rodent Models: Proteasome Inhibition is Not the Magic Answer[M]. University of California, Davis, 2014.
[3] Xu J H, Jiang Z, Solania A, et al. A commensal dipeptidyl aminopeptidase with specificity for N-terminal glycine degrades human-produced antimicrobial peptides in vitro[J]. ACS chemical biology, 2018, 13(9): 2513-2521.
[4] Maffioli E, Jiang Z, Nonnis S, et al. High-resolution mass spectrometry-based approaches for the detection and quantification of peptidase activity in plasma[J]. Molecules, 2020, 25(18): 4071.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Stock solution: Dissolve Glutaryl-Gly-Arg-AMC in DMSO to prepare a 10mM stock solution.
Note: After unused stock solution is aliquoted, store it at -20°C or -80°C in the dark to avoid repeated freezing and thawing.
(2) Working solution: Dilute the stock solution with experimental buffer (such as D-PBS) to the required working concentration, for example: 50μM[1].
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration by yourself. The working solution must be prepared and used immediately.
2. Determination of bovine trypsin activity using Glutaryl-Gly-Arg-AMC[1] (from the literature, for reference only)
(1) Reaction system: Add the following reagents in a 96-well plate in sequence: 50μL D-PBS (containing 2mM DTT), 50μL Glutaryl-Gly-Arg-AMC (final concentration 50μM), 50μL bovine trypsin (final concentration 100nM). Set up 3 replicate wells for each sample, and set up a blank control (no enzyme).
(2) Reaction conditions: Incubate the 96-well plate at 37°C for 30min.
(3) Fluorescence detection: Use a fluorescence microplate reader (excitation wavelength 360nm, emission wavelength 460nm) to detect fluorescence intensity. Record the change in fluorescence value and calculate the enzyme activity.
References:
[1] Xu J H, Jiang Z, Solania A, et al. A commensal dipeptidyl aminopeptidase with specificity for N-terminal glycine degrades human-produced antimicrobial peptides in vitro[J]. ACS chemical biology, 2018, 13(9): 2513-2521.
| Cas No. | 65147-16-2 | SDF | |
| Formula | C23H30N6O7 | M.Wt | 502.53 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9899 mL | 9.9497 mL | 19.8993 mL |
| 5 mM | 398 μL | 1.9899 mL | 3.9799 mL |
| 10 mM | 199 μL | 995 μL | 1.9899 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)