H-Tyr-His-OH |
Catalog No.GA22973
One-Click Copy Product Info
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H-Tyr-His-OH isa dipeptide that acts as an internal standard in LC-MS for analyzing the composition of histidine-containing dipeptides.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3788-44-1
Sample solution is provided at 25 µL, 10mM.
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H-Tyr-His-OH is a dipeptide that acts as an internal standard in LC-MS for analyzing the composition of histidine-containing dipeptides[1]. H-Tyr-His-OH serves as a nutritional supplement to improve cell viability, enhance cellular metabolic profiles, and reduce the production of lactate and ammonia during fed-batch culture processes[2]. H-Tyr-His-OH has been used as a model molecule to investigate the iontophoretic flux of different charged tyrosine-containing dipeptides[3].
In vivo, H-Tyr-His-OH treatment via intragastric administration at a dose of 10mg/kg daily for 7 days reduced blood pressure in spontaneously hypertensive rats[4].
References:
[1] Aldini G, Orioli M, Carini M, et al. Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry[J]. Journal of mass spectrometry, 2004, 39(12): 1417-1428.
[2] Kang S, Mullen J, Miranda L P, et al. Utilization of tyrosine‐and histidine‐containing dipeptides to enhance productivity and culture viability[J]. Biotechnology and bioengineering, 2012, 109(9): 2286-2294.
[3] Abla N, Naik A, Guy R H, et al. Effect of Charge and Molecular Weight on Transdermal Peptide Delivery by Iontophoresis: Abla, Naik, Guy, and Kalia[J]. Pharmaceutical research, 2005, 22(12): 2069-2078.
[4] Suetsuna K, Maekawa K, Chen J R. Antihypertensive effects of Undaria pinnatifida (wakame) peptide on blood pressure in spontaneously hypertensive rats[J]. The Journal of nutritional biochemistry, 2004, 15(5): 267-272.
| Animal experiment [1]: | |
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Animal models |
Spontaneously hypertensive rats |
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Preparation Method |
Spontaneously hypertensive rats (280-320g) were maintained in a controlled environment at 22±1°C and 60% relative humidity under a 12h light/dark cycle, and were given food and water ad libitum. After a 2-week adaptation period, rats were daily given the H-Tyr-His-OH (10mg/day) dissolved in 0.9% saline via gastric intubation for 7 days. The blood pressure was measured weekly for 3 weeks. |
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Dosage form |
10mg/kg/day; 7 days; i.g. |
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Applications |
H-Tyr-His-OH significantly lowered mean blood pressure (MBP), and diastolic blood pressure (DBP) in rats. |
References: [1] Suetsuna K, Maekawa K, Chen J R. Antihypertensive effects of Undaria pinnatifida (wakame) peptide on blood pressure in spontaneously hypertensive rats[J]. The Journal of nutritional biochemistry, 2004, 15(5): 267-272. | |
| Cas No. | 3788-44-1 | SDF | |
| Formula | C15H18N4O4 | M.Wt | 318.33 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1414 mL | 15.707 mL | 31.4139 mL |
| 5 mM | 628.3 μL | 3.1414 mL | 6.2828 mL |
| 10 mM | 314.1 μL | 1.5707 mL | 3.1414 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)