n-Octylpolyoxyethylene |
Catalog No.GA23310
One-Click Copy Product Info
|
n-Octylpolyoxyethylene is a nonionic surfactant that can dissolve membrane proteins and is used for protein solubilization, purification, and crystallization. Its critical micelle concentration (CMC) value is 6.6mM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 27252-75-1
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
n-Octylpolyoxyethylene is a nonionic surfactant that can dissolve membrane proteins and is used for protein solubilization, purification, and crystallization. Its critical micelle concentration (CMC) value is 6.6mM[1, 2]. The CMC value is defined as the minimum concentration at which surfactant molecules in a solvent associate to form micelles[3]. n-Octylpolyoxyethylene can be used as an antibacterial agent, possibly inhibiting bacterial growth by disrupting the bacterial cell membrane or interfering with the synthesis of proteins required for bacterial growth[4].
References:
[1] Hollingsworth K. The synthesis of a maltose responsive switch[D]. University of Leeds, 2015.
[2] Žuna K, Jovanović O, Khailova L S, et al. Mitochondrial uncoupling proteins (UCP1-UCP3) and adenine nucleotide translocase (ANT1) enhance the protonophoric action of 2, 4-dinitrophenol in mitochondria and planar bilayer membranes[J]. Biomolecules, 2021, 11(8): 1178.
[3] Hait S K, Moulik S P. Determination of critical micelle concentration (CMC) of nonionic surfactants by donor‐acceptor interaction with lodine and correlation of CMC with hydrophile‐lipophile balance and other parameters of the surfactants[J]. Journal of Surfactants and Detergents, 2001, 4(3): 303-309.
[4] Wang Q, Ko K S, Kapus A, et al. A spirochete surface protein uncouples store-operated calcium channels in fibroblasts: a novel cytotoxic mechanism[J]. Journal of Biological Chemistry, 2001, 276(25): 23056-23064.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Working solution: Dissolve n-Octylpolyoxyethylene in DMSO and dilute the stock solution with experimental buffer to the required working concentration.
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration yourself. The working solution must be prepared and used immediately.
2. Protocol for refolding, purifying and reconstructing proteins using n-Octylpolyoxyethylene[1] (Source literature, for reference only)
(1) Transform the expression plasmid containing the selected cDNA sequence into the Escherichia coli strain. After induction, high-pressure homogenization and centrifugation, the protein is separated into inclusion bodies.
(2) Dissolve 1mg of inclusion bodies in TE/G buffer containing 2% N-lauroylsarcosine, 1.3% Triton X-114, 0.3% n-Octylpolyoxyethylene, 1mM DTT and GTP (pH 7.5). Gradually mix in 50 mg of lipid mixture (DOPC:DOPE:CL, 45:45:10mol%).
(3) Concentrate the mixture and dialyze into the experimental buffer (50mM Na2SO4, 10mM MES, 10mM Tris and 0.6mM EGTA (pH 7.34)).
(4) Remove unfolded and aggregated proteins from the dialysate by centrifugation and a column containing hydroxyapatite. Use Bio-Beads SM-2 to remove nonionic detergents.
(5) Measure the protein concentration in the proteoliposomes using a protein assay kit. Verify protein purity by SDS-PAGE and silver staining.
Note: For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1]Žuna K, Jovanović O, Khailova L S, et al. Mitochondrial uncoupling proteins (UCP1-UCP3) and adenine nucleotide translocase (ANT1) enhance the protonophoric action of 2, 4-dinitrophenol in mitochondria and planar bilayer membranes[J]. Biomolecules, 2021, 11(8): 1178.
| Cas No. | 27252-75-1 | SDF | |
| Formula | (C2H4O)nC8H18O | M.Wt | |
| Solubility | Soluble in DMSO | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)