ROX NHS ester, 5-isomer |
Catalog No.GC59123
One-Click Copy Product Info
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ROX NHS ester, 5-isomer is a carboxy-X-rhodamine succinimidyl ester fluorescent dye (ex/em=575/600nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 209734-74-7; 344402-35-3
Sample solution is provided at 25 µL, 10mM.
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ROX NHS ester, 5-isomer is a carboxy-X-rhodamine succinimidyl ester fluorescent dye (ex/em=575/600nm). ROX NHS ester, 5-isomer is used in research involving its application as a key component for synthesizing specific fluorescent probes to detect the expression of target proteins[1].
References:
[1] Uddin MJ, Crews BC, Huda I, et al. Trifluoromethyl fluorocoxib a detects cyclooxygenase-2 expression in inflammatory tissues and human tumor xenografts. ACS Med Chem Lett. 2014 Jan 23;5(4):446-50.
Method for synthesizing the fluorescent probe CF3-fluorocoxib A using ROX NHS ester, 5-isomer[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Probe Synthesis: In anhydrous DMSO, react ROX NHS ester, 5-isomer with the amine derivative of CF3-indomethacin (in its free amine form) in the presence of triethylamine at 25°C for 16 hours to generate the target product, CF3-fluorocoxib A.
2. In Vivo Imaging: Administer the synthesized CF3-fluorocoxib A to mice via intraperitoneal (i.p.) injection at a dose of 1mg/kg. At the specified time point(s) post-injection, perform fluorescence imaging (ex/em=575/600nm) using an in vivo optical imaging system.
Precautions:
(1) The staining and measurement procedures should be performed in the dark to prevent fluorochrome quenching.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Uddin MJ, Crews BC, Huda I, et al. Trifluoromethyl fluorocoxib a detects cyclooxygenase-2 expression in inflammatory tissues and human tumor xenografts. ACS Med Chem Lett. 2014 Jan 23;5(4):446-50.
| Cas No. | 209734-74-7; 344402-35-3 | SDF | |
| Formula | C37H33N3O7 | M.Wt | 631.67 |
| Solubility | good in DMF, DMSO | Storage | -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5831 mL | 7.9155 mL | 15.8311 mL |
| 5 mM | 316.6 μL | 1.5831 mL | 3.1662 mL |
| 10 mM | 158.3 μL | 791.6 μL | 1.5831 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)