Cyanine5.5 maleimide |
Catalog No.GC59163
One-Click Copy Product Info
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Cyanine5.5 maleimide is a near-infrared fluorescent dye labeling reagent (Ex=673nm; Em=707nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1593644-50-8
Sample solution is provided at 25 µL, 10mM.
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Cyanine5.5 maleimide is a near-infrared fluorescent dye labeling reagent (Ex=673nm; Em=707nm). The maleimide group reacts with the SH-thiol group to form a thioether bond, thereby labeling proteins, peptides, antibodies, etc., containing cysteine molecules.
This plan is based on literature, please modify it to meet your specific needs.
1. Cyanine5.5 maleimide stock solution: Dissolve Cyanine5.5 maleimide dye in anhydrous DMSO to prepare a 10mM stock solution. Dissolve thoroughly by pipetting or vortexing.
Note: Long-term storage of the dye stock solution may reduce the activity of the dye; it should be used as soon as possible. It is recommended to aliquot the Cyanine5.5 maleimide stock solution and store it at -20℃ or -80℃ protected from light, avoiding repeated freeze-thaw cycles.
2. Protein stock solution: Dilute the protein to be labeled to 2mg/ml with PBS/HEPES, pH=7.5±0.5.
Note: (1) If the protein concentration is below 2mg/ml, the binding efficiency will be significantly reduced. For optimal labeling efficiency, a final protein concentration range of 2-10mg/ml is recommended. (2) The pH of the protein solution should be 7.5±0.5. Too low a pH will affect the labeling efficiency, while too high a pH may lead to non-specific labeling. (3) If your protein does not contain free cysteine, you need to treat the protein with a thiol reducing agent such as DTT or TCEP to generate thiol groups. Before coupling, remove free DTT from the protein solution by dialysis or gel filtration.
3. Calculate the volume of Cyanine5.5 maleimide and the protein to be labeled at a molar ratio of 5:1. Slowly add the Cyanine5.5 maleimide dye to the protein sample, gently shake to mix, and briefly centrifuge to collect the sample at the bottom of the reaction tube. Avoid vigorous mixing to prevent denaturation and inactivation of the protein sample.
Note: The amount of dye required for the labeling reaction depends on the protein properties. It is recommended to experiment within a molar ratio range of 2-10:1.
4. Place the reaction tube in a dark place and incubate gently at room temperature for 30-60 minutes.
5. Wash away the free dye using a desalting centrifuge column and PBS, and collect the protein conjugate.
| Cas No. | 1593644-50-8 | SDF | |
| Formula | C46H49ClN4O3 | M.Wt | 741.36 |
| Solubility | soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3489 mL | 6.7444 mL | 13.4887 mL |
| 5 mM | 269.8 μL | 1.3489 mL | 2.6977 mL |
| 10 mM | 134.9 μL | 674.4 μL | 1.3489 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)