Sulfo-Cyanine5-PEG3-Biotin |
Catalog No.GC59211
One-Click Copy Product Info
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Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.
Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.
Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.
The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.
| Cas No. | SDF | ||
| Formula | C48H65N6KO11S3 | M.Wt | 1037.36 |
| Solubility | good in water, DMF, DMSO | Storage | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 964 μL | 4.8199 mL | 9.6399 mL |
| 5 mM | 192.8 μL | 964 μL | 1.928 mL |
| 10 mM | 96.4 μL | 482 μL | 964 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)