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Guandine thiocyanate

Catalog No.GD13917 Copy One-Click Copy Product Info

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Guandine thiocyanate Chemical Structure

Cas No.: 593-84-0

Size Price Stock Qty
500g
$60.00
In stock

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Sample solution is provided at 25 µL, 10mM.



Description of Guandine thiocyanate

Guanidine thiocyanate is a strong protein denaturant and potent inhibitor of nucleases. Guanidinium thiocyanate is a nucleic acid protector in the extraction of DNA and RNA from cells. Guanidine thiocyanate is a common component of buffers used for nucleic acid extraction.

Guanidine thiocyanate based-nucleic acid extraction buffers can effectively inactivate high-consequence enveloped viruses, such as Ebola virus, Marburg virus, and Rift Valley Fever virus, as well as West Nile virus, influenza, Venezuelan equine encephalitis virus, and recently, SARS-CoV-2[1].
Guanidine thiocyanate-based nucleic acid extraction buffers are an effective means of inactivating poliovirus infectious materials and potentially infectious materials[1].

[1]. Michelle J Honeywood, et al. Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials. J Virol Methods. 2021 Nov:297:114262.  

Chemical Properties of Guandine thiocyanate

Cas No. 593-84-0 SDF
Formula C2H6N4S M.Wt 118.16
Solubility DMSO : 100 mg/mL (846.31 mM; Need ultrasonic); H2O : 100 mg/mL (846.31 mM; Need ultrasonic) Storage Store at RT
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Guandine thiocyanate

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 8.4631 mL 42.3155 mL 84.631 mL
5 mM 1.6926 mL 8.4631 mL 16.9262 mL
10 mM 846.3 μL 4.2316 mL 8.4631 mL
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

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In vivo Formulation Calculator (Clear solution) of Guandine thiocyanate

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % saline
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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