Trypan Blue Stain solution(0.4%) |
Catalog No.GF00376
One-Click Copy Product Info
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Trypan Blue, also known as Diphenyl Blue, is a non-permeable dye used to assess the integrity of cell membranes and cell viability.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Trypan Blue, also known as Diphenyl Blue, is a non-permeable dye commonly used to assess cell membrane integrity and cell viability. It is utilized for identifying dead cells in tissue and cell cultures. Cells that have lost their activity or have compromised cell membranes can be stained blue with Trypan Blue. Loss of cell membrane integrity is often indicative of cell death. Following Trypan Blue staining, cell viability can be quantified with precision through direct counting under a microscope or by counting images taken under a microscope.
Trypan Blue can be engulfed by macrophages, making it suitable for in vivo staining of these cells. Additionally, Trypan Blue resistance has been observed in apoptotic bodies.
This product is a 0.4% Trypan Blue storage solution prepared using 0.85% NaCl. The storage solution is recommended to be aliquoted and stored at 4°C in the dark, with a shelf life of one year.
Preparation of Working Solution
Dilute the storage solution with serum-free cell culture medium or PBS to prepare a 0.04% Trypan Blue working solution.
Note: Adjust the concentration of the Trypan Blue working solution according to experimental requirements,preparing it just before use.
Cell Staining
1.For suspension cells, collect the cells by centrifugation, wash them twice with PBS, each time for 5 minutes.
For adherent cells, remove the culture medium, add trypsin to digest the cells. After centrifugation, remove the supernatant, and wash the cells twice with PBS, each time for 5 minutes.
2.Add 1 mL of the Trypan Blue working solution and incubate at room temperature for 5 minutes.
3.Centrifuge at 400g, 4°C for 3-4 minutes, discard the supernatant, and wash the cells twice with PBS, each time for 5 minutes.
4.Resuspend the cells in 1 mL of serum-free culture medium or PBS. Calculate cell viability by directly counting under a microscope or by counting images taken under a microscope.
Cell Viability (%) = (Total Live Cells) / (Total Live Cells + Total Dead Cells) * 100%
Notes
1.Avoid excessively long staining times as live cells may accumulate dye over time, resulting in lower detection results.
2.If there is precipitation in the dye solution before staining, filter it to remove the precipitate before use.
3.There is potential carcinogenic risk; for your safety and health, wear lab coats and disposable gloves while handling.
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