TCEP Solution (0.5M in Water) |
Catalog No.GF02960
One-Click Copy Product Info
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TCEP Solution (0.5M in Water) is a trialkylphosphine reagent that selectively reduces protein disulfides and promotes NF-κB-DNA binding.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5961-85-3
Sample solution is provided at 25 µL, 10mM.
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TCEP Solution (0.5M in Water) is a trialkylphosphine reagent that selectively reduces protein disulfides and promotes NF-κB-DNA binding [1]. Through the strong selective reduction for the disulfide bonding of wool fibers, TCEP Solution could improve the adsorption amount and rate of dyes on the fiber at low dyeing temperatures (80°C) [2]. TCEP Solution can act as a reductant that completely reduces dehydroascorbic acid into ascorbic acid at pH 1.5 [3]. TCEP Solution has been widely used in protein purification experiments and for facilitating the highly specific cleavage of the C-terminal aspartic acid residues of proteins[4].
References:
[1] Dieguez-Acuña F J, Woods J S. Inhibition of NF-κB–DNA binding by mercuric ion: utility of the non-thiol reductant, tris (2-carboxyethyl) phosphine hydrochloride (TCEP), on detection of impaired NF-κB–DNA binding by thiol-directed agents[J]. Toxicology in vitro, 2000, 14(1): 7-16.
[2] Shen J, Gao P, Ma H. The effect of tris (2-carboxyethyl) phosphine on the dyeing of wool fabrics with natural dyes[J]. Dyes and Pigments, 2014, 108: 70-75.
[3] Sato Y, Uchiki T, Iwama M, et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris (2-carboxyethyl) phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution[J]. Biological and Pharmaceutical Bulletin, 2010, 33(3): 364-369.
[4] McCracken D S, Foreman D J, Esposito K G, et al. TCEP‐Mediated Protein Hydrolysis: Highly Selective Cleavage C‐Terminal to Aspartic Acid[J]. Rapid Communications in Mass Spectrometry, 2026, 40(10): e70051.
| Cas No. | 5961-85-3 | SDF | |
| Formula | M.Wt | ||
| Solubility | Storage | 2-8°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Appearance: A solution in water
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)