Penicillin-Streptomycin (100×) |
Catalog No.GF04868
One-Click Copy Product Info
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Penicillin-Streptomycin (100×), sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Penicillin-Streptomycin (100×), sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin interferes with the final stage of synthesis of the bacterial cell wall. Streptomycin inhibits elongation at transpeptidation step during protein synthesis, and binds to 30S subunit, causing misreading.
Penicillin-Streptomycin (100×) is a liquid formulation which contains 10,000 units/mL of penicillin and 10 mg/mL of streptomycin in 0.9% NaCl. Common working concentration for penicillin is 100 units/mL, for streptomycin is 100 μg/mL.
Usage Instructions
Penicillin-Streptomycin Solution (100×) can be used following one of these two methods:
1.Direct addition to sterile cell culture medium:
Add the Penicillin-Streptomycin Solution (100×) to the cell culture medium at a ratio of 5 mL per 500 mL of medium. Mix well before use.
2.Addition during the preparation of the cell culture medium, followed by filtration for sterilization:
Add the Penicillin-Streptomycin Solution (100×) to the medium at a ratio of 10 mL per 1 L of medium prepared. After preparation, filter to sterilize before use.
Precautions
1.Minimize the number of freeze-thaw cycles to prevent a decrease in potency.
2.Ensure sterile handling to avoid contamination.
3.This product is for scientific research by professionals only and should not be used for clinical diagnosis or treatment, nor in food or drugs.
4.For your safety and health, please wear a lab coat and disposable gloves during handling.
| Cas No. | SDF | ||
| Formula | M.Wt | ||
| Solubility | Storage | Stored at -20°C, and is stable for 1 year. | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)