Hyaluronidase |
Catalog No.GC19790
One-Click Copy Product Info
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Hyaluronidase is a natural enzyme that can degrade hyaluronic acid and has high specificity and hydrolytic activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 37326-33-3;9001-54-1
Sample solution is provided at 25 µL, 10mM.
Hyaluronidase is a natural enzyme that can degrade hyaluronic acid and has high specificity and hydrolytic activity[1]. The mechanism of action of hyaluronidase is mainly to reduce the viscosity and molecular weight of hyaluronic acid by cutting the β-1,4-glucosidic bond of hyaluronic acid[2]. In humans, six different hyaluronidases have been identified, HYAL 1-4, HYAL-P1 and PH-20, of which PH-20 has the strongest biological activity[3]. Hyaluronidase is often used to improve the tissue permeability of drugs, promote drug absorption, and can also reverse adverse reactions caused by hyaluronic acid fillers[4].
In vitro, hyaluronidase (100mU/ml) treatment of astrocytes that knocked down CD44 expression for 24 hours enhanced the activity of Rac1, causing the cells to become "star-like" morphology[5]. Hyaluronidase (12U/mL) treats MM6 cells, directly targeting HA and eliminating the HA signal[6].
In vivo, hyaluronidase treatment of lymphedema model mice rapidly reduced the size of the edema area and enhanced lymphangiogenesis in the edematous limbs [7].
References:
[1] Menzel E J, Farr C. Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses[J]. Cancer letters, 1998, 131(1): 3-11.
[2] Tao L, Song F, Xu N, et al. New insights into the action of bacterial chondroitinase AC I and hyaluronidase on hyaluronic acid[J]. Carbohydrate polymers, 2017, 158: 85-92.
[3] Weber G C, Buhren B A, Schrumpf H, et al. Clinical applications of hyaluronidase[J]. Therapeutic Enzymes: Function and Clinical Implications, 2019: 255-277.
[4] Vartanian A J, Frankel A S, Rubin M G. Injected hyaluronidase reduces restylane-mediated cutaneous augmentation[J]. Archives of Facial Plastic Surgery, 2005.
[5] Konopka A, Zeug A, Skupien A, et al. Cleavage of hyaluronan and CD44 adhesion molecule regulate astrocyte morphology via Rac1 signalling[J]. PloS one, 2016, 11(5): e0155053.
[6] Zahreddine H A, Culjkovic-Kraljacic B, Emond A, et al. The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity[J]. Elife, 2017, 6: e29830.
[7] Roh K, Cho S, Park J, et al. Therapeutic effects of hyaluronidase on acquired lymphedema using a newly developed mouse limb model[J]. Experimental Biology and Medicine, 2017, 242(6): 584-592.
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Cell experiment [1]: |
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Cell lines |
Astrocytes |
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Preparation method |
Astrocytes cultured in vitro were starved overnight in medium without serum and then incubated for 24h with hyaluronidase type IV-S (100mU/ml), heat-inactivated hyaluronidase or deionized sterile water. |
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Reaction Conditions |
100mU/ml; 24h |
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Applications |
Hyaluronidase treatment, as well as knockdown of CD44, in astrocytes result in a “stellate”-like morphology. |
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References: |
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| Cas No. | 37326-33-3;9001-54-1 | SDF | |
| Formula | M.Wt | ||
| Solubility | Storage | Store at -20°C,protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: ≥300U/mg Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)